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Transcription profiling of mouse lung from individuals with airway hyperactivity and goblet cell metaplasia post-Sendai virus infection

ABSTRACT: Mice representing phenotypic extremes of airway hyperreactivity and goblet cell metaplasia post-Sendai virus infection were identified from a 500 mouse F2 cohort (CB6F2/J). Whole lung RNA from 3 mice at each extreme was analyzed via microarray for gene expression. Subsequent pairwise comparisons between arrays allowed the identification of genes differentially expressed with respect to the disease phenotypes (airway hyperreactivity and goblet cell metaplasia).

ORGANISM(S): Mus musculus

SUBMITTER: Anand Champak Patel

PROVIDER: E-GEOD-3925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic segregation of airway disease traits despite redundancy of calcium-activated chloride channel family members.

Patel Anand C AC Morton Jeffrey D JD Kim Edy Y EY Alevy Yael Y Swanson Suzanne S Tucker Jennifer J Huang Guaming G Agapov Eugene E Phillips Thomas E TE Fuentes Maria E ME Iglesias Antonio A Aud Dee D Allard John D JD Dabbagh Karim K Peltz Gary G Holtzman Michael J MJ

Physiological genomics 20060328 3

Complex airway diseases such as asthma and chronic obstructive pulmonary disease exhibit stereotyped traits (especially airway hyperreactivity and mucous cell metaplasia) that are variably expressed in each patient. Here, we used a mouse model for virus-induced long-term expression of these traits to determine whether individual traits can be genetically segregated and thereby linked to separate determinants. We showed that an F2 intercross population derived from susceptible and nonsusceptible ...[more]

PMID: 16569774

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