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Comparison of gene expression between MEFs and induced embryonic Sertoli-like cells

ABSTRACT: To compare the the genomic profile of MEFs and induced embryonic Sertoli-like cells following 2 weeks of dox withdrawal. ieSCs (from MEFs and from TTFs) and MEF control were grown in F12/DMEM medium without dox for two weeks.

ORGANISM(S): Mus musculus

SUBMITTER: Albert Cheng

PROVIDER: E-GEOD-39650 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Direct reprogramming of fibroblasts into embryonic Sertoli-like cells by defined factors.

Buganim Yosef Y Itskovich Elena E Hu Yueh-Chiang YC Cheng Albert W AW Ganz Kibibi K Sarkar Sovan S Fu Dongdong D Welstead G Grant GG Page David C DC Jaenisch Rudolf R

Cell stem cell 20120901 3

Sertoli cells are considered the "supporting cells" of the testis that play an essential role in sex determination during embryogenesis and in spermatogenesis during adulthood. Their essential roles in male fertility along with their immunosuppressive and neurotrophic properties make them an attractive cell type for therapeutic applications. Here we demonstrate the generation of induced embryonic Sertoli-like cells (ieSCs) by ectopic expression of five transcription factors. We characterize the ...[more]

PMID: 22958931

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Comparison of gene expression between MEFs and induced embryonic Sertoli-like cells

Project description:To compare the the genomic profile of MEFs and induced embryonic Sertoli-like cells following 2 weeks of dox withdrawal.

2012-12-20 | GSE39650 | GEO

Gene expression comparison between different stages of endogenous Sertoli cells and induced embryonic Sertoli cells derived from MEFs and TTFs

Project description:To compare the transcriptional profile of endogenous Sertoli cells from different Stage of Sertoli cell development (embryonic, immature, mature) to the transcriptionla profile of induced embryonic Sertoli cells derived from MEFs or TTFs we employed the agilent whole genome microarray Keywords: Expression profiling by array The following samples were analyzed in duplicates (MEFs, TTFs, ieSCs (derived from MEFs), ieSCs (derived from TTFs), 14.5 dpc male gonad, immature Sertoli (19 dpc embryo testis) and mature (8 week-old mouse testis))

2012-12-20 | E-GEOD-39648 | biostudies-arrayexpress

Comparison of gene expression between MEFs, MEFs (NWD), immature Sertoli cells and mature Sertoli cells

Project description:To compare the the genomic profile of MEFs, immature Sertoli, mature Sertoli cells, and MEFs (NWD) after infection of Nr5a1, Wt1 and Dmrt1 after 1 month of dox exposure.

2012-12-20 | GSE39649 | GEO

Comparison of gene expression between MEFs, MEFs (NWD), immature Sertoli cells and mature Sertoli cells

Project description:To compare the the genomic profile of MEFs, immature Sertoli, mature Sertoli cells, and MEFs (NWD) after infection of Nr5a1, Wt1 and Dmrt1 after 1 month of dox exposure. MEFs (NWD) were infected with Nr5a1, Wt1 and Dmrt1 and were exposed to dox for 1 month. MEFs, immature Sertoli and mature Sertoli cells were cultured for 3 days and collected

2012-12-20 | E-GEOD-39649 | biostudies-arrayexpress

Gene expression comparison between different stages of endogenous Sertoli cells and induced embryonic Sertoli cells derived from MEFs and TTFs

2012-12-20 | GSE39648 | GEO

Whole genome analysis of Sertoli cell gene expression by retinoblastoma-1

Project description:Analysis of the regulation of gene expression profiles by retinoblastoma-1 in Sertoli cells. Conditional knockout of Rb1 in Sertoli cells led to progressive infertiliy in male mice that occured between 10 and 14 weeks of age. Results of gene expression studies performed on 6 week-old purified Sertoli cells helped elucidate the key role of RB1 in mature, differentiated Sertoli cells.

2009-09-02 | GSE17904 | GEO

Gene expression profiling of MEFs with and without Mitf induction compared to wild type MEF and primaryt melanocytes

Project description:We performed a global mRNA expression profiling via RNA-seq. Genes differentially expressed in Mitf knock-in MEFs compared to vehicle-treated knock-in MEFs were clustered and compared to expression in untreated wild-type MEFs and primary mouse melanocytes. This clustering demonstrates a gradual transition in the expression profile of Dox-treated Mitf knock-in MEFs toward the melanocyte expression profile

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Mus musculus

Project description:Gene expression comparison between different stages of endogenous Sertoli cells and induced embryonic Sertoli cells derived from MEFs and TTFs

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Mouse sertoli cells Palmitoylated protein MS

Project description:Elevated palmitic acid, a most abundant saturated fatty acid, is a risk factor for obesity complications. Here, we report that palmitic acid impairs spermatogenesis by inducing abnormal palmitoylation in Sertoli cells. Firstly, we proposed a correlation between palmitic acid and spermatogenesis by reporting a significant elevation of serum palmitic acid levels in patients with dyszoospermia. In palmitic acid -treated mice, the blood-testis barrier was found to be disrupted, and decreased tight junction protein expression was observed in palmitic acid-treated Sertoli cells. Palmitic acid entered the endoplasmic reticulum and induced endoplasmic reticulum stress, resulting in damage to Sertoli cell barriers.

2022-08-12 | PXD028778 | Pride

Expression data throughout reprogramming of MEF to iPS using a Dox-inducible promoter

Project description:6C secondary MEFs were treated with Dox in mES media to turn on the Oct4, Klf4, cMyc, Sox2. Total RNA was extracted at day 0 (no Dox), day2, 5, 8, 11, 16 and 21 (with Dox) and day 30 (Dox-independent secondary iPS). RNA from Parental MEFs and Primary-iPS cells were also extracted for reference. 1B secondary MEFs were Dox treated for 5-days followed by RNA extraction. Subsequently, a culture removed of Dox treatment for an additional 5days was also analyzed.

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