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Transcription factor occupancy is linked to DNA methylation turnover at active regulatory regions [Bisulfite-Seq]

ABSTRACT: Genome wide analysis revealed that distal regulatory elements form Low Methylated Regions (LMRs). Even though transcription factor binding is required for LMR formation, we show for the test case CTCF that actual occupancy does not distinguish DNA methylation states. However, in line with a dynamic model of binding and DNA methylation turnover, we find that the product of active demethylation, 5-hydroxymethylcytosine (5hmC), is enriched at LMRs. 5hmC is present at active regulatory regions in stem and somatic cells and as a result a substantial fraction of changes in 5hmC occurs at LMRs. This suggests that transcription factor binding mediates active turnover of DNA methylation as an integral part of reprogramming of regulatory regions. CTCF ChIP bisulfite sequencing in mouse embryonic stem cells and whole genome shotgun bisulfite sequencing of RESTko mouse embryonic stem (ES) cells

ORGANISM(S): Mus musculus

SUBMITTER: Dirk Schuebeler

PROVIDER: E-GEOD-39736 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription factor occupancy is linked to DNA methylation turnover at active regulatory regions

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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2012-01-06 | E-GEOD-32631 | biostudies-arrayexpress

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2012-07-30 | E-GEOD-38041 | biostudies-arrayexpress

HEK 293T cells HELP-tagging cytosine methylation data treated with 5-aza-2-deoxycytidine

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2015-02-13 | E-GEOD-62592 | biostudies-arrayexpress

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