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Alteration of gene expression in differentiating C2C12 cells upon inhibition of PCAF by embelin

ABSTRACT: KAT2B (PCAF) belongs to GNAT family of lysine acetyltransferases (KAT), which specifically acetylates histone H3K9 residue and several nonhistone proteins. PCAF is also a transcription coactivator. Due to the lack of PCAF, KAT specific small molecule inhibitor, the exclusive role of the acetyltransferase activity of PCAF is not well understood. Here we report that a hydroxy benzoquinone class of natural compound, embelin, specifically inhibits H3Lys9 acetylation in mice and recombinant PCAF mediated acetylation with near complete specificity in vitro. Further, using embelin, we have identified the PCAF regulated gene networks during muscle differentiation, further highlighting the broader regulatory functions of PCAF in muscle differentiation, apart from regulation through MyoD acetylation. C2C12 cells were treated with either DMSO or 10uM embelin in GM/DM for either 24 or 48h.

ORGANISM(S): Mus musculus

SUBMITTER: Tapas Kundu

PROVIDER: E-GEOD-39827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Alteration of gene expression in HEK 293T cells upon inhibition of PCAF by embelin

Project description:KAT2B (PCAF) belongs to GNAT family of lysine acetyltransferases (KAT), which specifically acetylates histone H3K9 residue and several nonhistone proteins. PCAF is also a transcription coactivator. Due to the lack of PCAF, KAT specific small molecule inhibitor, the exclusive role of the acetyltransferase activity of PCAF is not well understood. Here we report that a hydroxy benzoquinone class of natural compound, embelin, specifically inhibits H3Lys9 acetylation in mice and recombinant PCAF mediated acetylation with near complete specificity in vitro. Further, using embelin, we have identified the PCAF regulated gene networks during muscle differentiation, further highlighting the broader regulatory functions of PCAF in muscle differentiation, apart from regulation through MyoD acetylation. HEK 293T cells were treated with DMSO (solvent control) and 20uM embelin for 24h. Cells were harvested and total RNA was extracted by Trizol method. RNA was cleaned up using RNeasy MinElute Cleanup Kit (Qiagen) and reverse transcribed, labelled and subsequently hybridized to Agilent human 4X 44k array slide.

2013-03-31 | E-GEOD-39828 | biostudies-arrayexpress

Alteration of gene expression in HEK 293T cells upon inhibition of PCAF by embelin

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Alteration of gene expression in differentiating C2C12 cells upon inhibition of PCAF by embelin

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Alteration of gene expression in two cell lines upon inhibition of PCAF by embelin

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2013-03-31 | E-GEOD-39829 | biostudies-arrayexpress

PCAF/GCN5-regulated genes in MEFs

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Distribution of p27- and PCAF binding sites (BS) on the chromatin of HCT116 cell line

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Gcn5 and PCAF negatively regulate interferon β production through HAT-independent inhibition of TBK1 [RNA-Seq]

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PALB2 acetylation by KAT2A/B (GCN5/PCAF) in vitro

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2023-03-10 | PXD014681 | Pride

Gcn5 and PCAF negatively regulate interferon β production through HAT-independent inhibition of TBK1 [RNA-Seq]

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