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The RALA pathway can maintain the proliferation of KRAS- and BRAF-mutated cancer cells

ABSTRACT: By silencing of RALA, a downstream member of the RAS signal transduction pathway, we aimed to determine whether genes downstream of a mutated KRAS (codon 12 or 13) or a mutated BRAF can have significant functions in colorectal cancer carcinogenesis. RALA was silenced in three colorectal cancer cell lines (SW480, HCT116 and HT29). Effects were normalized to mock-transfected cells and the effects of scramble siRNA were excluded. SW480, HCT116 and HT29 cell lines were treated with the PI3K inhibitor LY294002 or DMSO.

ORGANISM(S): Homo sapiens

SUBMITTER: Balazs Györffy

PROVIDER: E-GEOD-39857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of RAL signal transduction in KRAS- and BRAF-mutated cells and prognostic potential of the RAL signature in colorectal cancer.

Győrffy Balázs B Stelniec-Klotz Iwona I Sigler Christian C Kasack Katharina K Redmer Torben T Qian Yu Y Schäfer Reinhold R

Oncotarget 20150501 15

Our understanding of oncogenic signaling pathways has strongly fostered current concepts for targeted therapies in metastatic colorectal cancer. The RALA pathway is novel candidate due to its independent role in controlling expression of genes downstream of RAS.We compared RALA GTPase activities in three colorectal cancer cell lines by GTPase pull-down assay and analyzed the transcriptional and phenotypic effects of transient RALA silencing. Knocking-down RALA expression strongly diminished the ...[more]

PMID: 26033452

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