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Role of interleukin-4 in the licensing of dendritic cells for the induction of Th2 responses

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Juan Oliveros

PROVIDER: E-GEOD-39863 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-4 blocks TH1-polarizing/inflammatory cytokine gene expression during monocyte-derived dendritic cell differentiation through histone hypoacetylation.

López-Bravo María M Minguito de la Escalera María M Domínguez Pilar M PM González-Cintado Leticia L del Fresno Carlos C Martín Pilar P Martínez del Hoyo Gloria G Ardavín Carlos C

The Journal of allergy and clinical immunology 20131017 6

<h4>Background</h4>Whereas recent research has characterized the mechanism by which dendritic cells (DCs) induce T(H)1/T(H)17 responses, the functional specialization enabling DCs to polarize T(H)2 responses remains undefined. Because IL-4 is essential during T(H)2 responses not only by acting on CD4(+) T cells through the activation of GATA-3 but also by regulating IgE class-switching, epithelial cell permeability, and muscle contractility, we hypothesized that IL-4 could also have a role in th ...[more]

PMID: 24139608

Similar Datasets

Role of interleukin-4 in the licensing of dendritic cells for the induction of Th2 responses [B]

Project description:T helper type 2 (Th2) responses are crucial for defense against infections by helminths and are responsible for the development of allergic reactions that can lead to severe clinical disorders, such as asthma or anaphylaxis, and ultimately to death. The induction of Th2 responses requires a specific activation process, triggered by specialized dendritic cells (DCs), by which naive CD4+ Th0 cells acquire the capacity to produce Th2 cytokines. However, the mechanistic basis of the functional specialization enabling DCs for the initiation of Th2 responses has remained elusive. Here we show that interleukin-4 (IL-4), a cytokine produced by basophils, mast cells and Th2-polarized CD4+ T helper cells, exerting a crucial function during anti-helminths and allergic Th2 responses, has a key role in the licensing/conditioning of DCs for the induction of Th2 responses, by bloking their potential to produce Th1-driving cytokines, such as IL-12, IL-18 and IL-23. Microarray analyses (duplicates) were used to compared the transcriptional profile of monocyte-derived dendritic cells (moDCs) cultured with GM-CSF in the absence or presence of interleukin-4: IL4-moDCs versus C-moDCs.

2014-02-19 | E-GEOD-39858 | biostudies-arrayexpress

Role of interleukin-4 in the licensing of dendritic cells for the induction of Th2 responses [A]

Project description:T helper type 2 (Th2) responses are crucial for defense against infections by helminths and are responsible for the development of allergic reactions that can lead to severe clinical disorders, such as asthma or anaphylaxis, and ultimately to death. The induction of Th2 responses requires a specific activation process, triggered by specialized dendritic cells (DCs), by which naive CD4+ Th0 cells acquire the capacity to produce Th2 cytokines. However, the mechanistic basis of the functional specialization enabling DCs for the initiation of Th2 responses has remained elusive. Here we show that interleukin-4 (IL-4), a cytokine produced by basophils, mast cells and Th2-polarized CD4+ T helper cells, exerting a crucial function during anti-helminths and allergic Th2 responses, has a key role in the licensing/conditioning of DCs for the induction of Th2 responses, by bloking their potential to produce Th1-driving cytokines, such as IL-12, IL-18 and IL-23. Microarray analyses (duplicates) were for two types of comparisons: 1. moDCs stimulated with LPS from Escherichia coli versus C-moDCs non stimulated (control). 2. moDCs stimulated with LPS from Escherichia coli in presence of IL4 versus C-moDCs non stimulated (control).

2014-02-19 | E-GEOD-39862 | biostudies-arrayexpress

Transcriptome profiles of brain glioma stem cells and normal neural stem cells

Project description:A human cDNA microarray comprising 29,187 human genome probes was used to evaluate the transcriptional changes between brian glioma stem cells (GSC) and normal neural stem cells(NSC). There were 6 samples that were analyzed, 3 samples were from glioma stem cells and the other 3 samples form normal neural stem cells were controls. cell type: brain glioma stem cells:GSC_H004, GSC_H005, GSC_H006 ; cell line: normal neural stem cells: NSC_H001, NSC_H002, NSC_H003 biological replicate: NSC_H001, NSC_H002, NSC_H003; biological replicate: GSC_H004, GSC_H005, GSC_H006

2014-07-30 | E-GEOD-41031 | biostudies-arrayexpress

Project description:In this study we show that the Arabidopsis transcription factor MYB46, previously described to regulate secondary cell wall biosynthesis in the vascular tissue of the stem, is pivotal for mediating disease susceptibility to the fungal pathogen Botrytis cinerea. We identified MYB46 by its ability to bind to a new cis element located in the 5Â´ promoter region of the pathogen-induced Ep5C gene which encodes a type III cell wall-bound peroxidase. We present genetic and molecular evidence indicating that MYB46 modulates the magnitude of Ep5C gene induction following pathogenic insults. Moreover, we demonstrate that different myb46 knock-down mutant plants exhibit increased disease resistance to B. cinerea, a phenotype that is accompanied by selective transcriptional reprogramming of a set of genes encoding cell wall proteins and enzymes, of which extracellular type III peroxidases are conspicuous. In essence our results substantiates that defense-related signaling pathways and cell wall integrity are interconnected, and MYB46 likely functions as a disease susceptibility modulator to B. cinerea through the integration of cell wall remodeling and downstream activation of secondary lines of defense. 10-15 days-old plants were collected, frozen in liquid nitrogen and stored at -80ÂºC. Independent replicates from 3 different experiments (separated in time) were used in the analysis. Samples from Col-0 and myb46-2 were compared.

2011-11-21 | E-GEOD-25838 | biostudies-arrayexpress

Transcription profiling of mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells (HSCs), progenitor cells (HPCs), and granulocytes somatic cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer

Project description:Since the creation of Dolly, the first sheep cloned via somatic cell nuclear transfer (SCNT), in 1997, more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1-5%) is that the clones are likely derived from adult stem cells, which form an extremely small fraction in most adult tissues. Support for this hypothesis is that the cloning efficiency of full term development using embryonic stem (ES) cells as nuclear donors is 5-10 times higher than that for somatic cells as nuclear donors. Additionally, cloned pups could not be produced directly from cloned embryos derived from nuclei of differentiated B and T cells or neuronal cells. The question remains: can SCNT-derived animal clones be derived from truly differentiated somatic cells? We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells (HSCs), progenitor cells (HPCs), and granulocytes. Surprisingly, we found that cloning efficiency increases over the differentiation hierarchy. The terminally differentiated post-mitotic granulocytes yielded the greatest cloning efficiency and we produced two cloned pups from granulocytes. We conclude that cloned mammals could be directly derived from post-mitotic differentiated somatic cells. Experiment Overall Design: single channel Affymetrix arrays to confirm cell-type specific gene expression profiles

2008-06-14 | E-GEOD-5677 | biostudies-arrayexpress

Col_Rose Bengal versus eto1-14_Rose Bengal

Project description:Rose Bengal-treated wild-type seedlings versus Rose Bengal-treated eto1-14 mutant

2011-03-01 | E-MEXP-3011 | biostudies-arrayexpress

Col_Rose Bengal versus lox1lox5_Rose Bengal

Project description:Rose Bengal-treated wild type seedlings versus Rose Bengal-treated lox1lox5 mutant

2011-03-01 | E-MEXP-3010 | biostudies-arrayexpress

The previously uncharacterized HCMV protein pUL13 increases mitochondrial bioenergetics during infection

Project description:HCMV is a prevalent human pathogen which has been shown to globally alter cellular metabolism during its replication cycle, increasing central carbon metabolism, lipid metabolism, and OXPHOS. Here we demonstrate that the previously uncharacterized viral protein pUL13 is required for productive viral replication and sufficient to increase OXPHOS during infection.

2021-07-19 | PXD020009 | Pride

Col versus Col_Rose Bengal

Project description:MS treated wild-type Arabidopsis seedlings versus MS Rose Bengal (RB)-containing medium (10 microM) wild type treated seedlings

2011-03-01 | E-MEXP-3009 | biostudies-arrayexpress

Genetic Analysis of opaque 2 Modifier Loci in Quality Protein Maize

Project description:Quality Protein Maize (QPM) was created by selecting for genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs. Compared 18 days after pollination endosperm transcript profiles in true breeding modified (vitreous; V) and non-modified (opaque; O) opaque 2 kernels. Used four vitreous biological reps (V1-V4) and four opaque biological reps (O1-O4). Each biological rep is a pool of 25 kernels representing 5 kernels form each of 5 uniform phenotype ears (vitreous or opaque). Four arrays used: Array 1= V1 vs O1, Array 2 = V2 vs O2, Array 3 = V3 vs O3, Array 4 = V4 vs O4

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