Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

TGF-beta/Smad2/3 signaling directly regulates several miRNAs in mouse ES Cells and early embryos

ABSTRACT: Purpose: We aimed to identify miRNAs which are induced by the Activin/Nodal effectors, P-Smad2/3, in order to further our understanding of how P-Smad2/3 controls downstream gene expression in mouse ES cells to regulate crucial biological processes. Methods: We used a previously developed Tetracycline-On (Tet-On) system (TAG1) to manipulate the levels of P-Smad2/3 in mouse ES cells and performed an Illumina deep-sequencing screen to identify miRNAs which followed the P-Smad2/3 pathway. Results: We filtered the deep-seq data to identify a list of 28 miRNAs which showed a >1.25 fold increase in response to P-Smad2/3 induction and a >1.25 fold decrease in response to P-Smad2/3 repression. Conclusions: Our study represents a comprehensive global profiling of miRNA expression in response to changes in P-Smad2/3 levels in mouse ES cells. miRNA profiles of TAG1 cells which were untreated (control), SB-431541 treated (P-Smad2/3 repressed), or Dox treated (P-Smad2/3 induced), were generated using Illumina GAII.

ORGANISM(S): Mus musculus

SUBMITTER: Carme Camps

PROVIDER: E-GEOD-39994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

TGF-β/Smad2/3 signaling directly regulates several miRNAs in mouse ES cells and early embryos.

Redshaw Nicholas N Camps Carme C Sharma Vikas V Motallebipour Mehdi M Guzman-Ayala Marcela M Oikonomopoulos Spyros S Thymiakou Efstathia E Ragoussis Jiannis J Episkopou Vasso V

PloS one 20130130 1

The Transforming Growth Factor-β (TGF-β) signaling pathway is one of the major pathways essential for normal embryonic development and tissue homeostasis, with anti-tumor but also pro-metastatic properties in cancer. This pathway directly regulates several target genes that mediate its downstream functions, however very few microRNAs (miRNAs) have been identified as targets. miRNAs are modulators of gene expression with essential roles in development and a clear association with diseases includi ...[more]

PMID: 23390484

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Dataset Information

TGF-beta/Smad2/3 signaling directly regulates several miRNAs in mouse ES Cells and early embryos

Publications

TGF-β/Smad2/3 signaling directly regulates several miRNAs in mouse ES cells and early embryos.

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