Unknown,Transcriptomics,Genomics,Proteomics

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Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival


ABSTRACT: Here we show that the phytochrome-less chlorophyte Chlamydomonas reinhardtii retains a functional pathway to synthesize the linear tetrapyrrole (bilin) precursor of the phytochrome chromophore. Reverse genetic, metabolic inactivation and bilin rescue experiments establish that this pathway is needed for heme iron acquisition and for the diurnal transition to phototrophic growth. RNA-Seq measurements reveal a bilin-dependent signaling network that is necessary for the heterotrophic to phototrophic transition. These results imply the presence of a novel bilin sensor pathway that may be widely distributed amongst oxygenic photosynthetic organisms. We isolated RNA from heterotrophic suspension cultures of 4A+ WT and the hmox1 mutant grown in the presence or absence of 0.1 mM BV IXM-NM-1 before and after transfer to low light.

ORGANISM(S): Chlamydomonas reinhardtii

SUBMITTER: Sabeeha Merchant 

PROVIDER: E-GEOD-40031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival.

Duanmu Deqiang D   Casero David D   Dent Rachel M RM   Gallaher Sean S   Yang Wenqiang W   Rockwell Nathan C NC   Martin Shelley S SS   Pellegrini Matteo M   Niyogi Krishna K KK   Merchant Sabeeha S SS   Grossman Arthur R AR   Lagarias J Clark JC  

Proceedings of the National Academy of Sciences of the United States of America 20130123 9


The maintenance of functional chloroplasts in photosynthetic eukaryotes requires real-time coordination of the nuclear and plastid genomes. Tetrapyrroles play a significant role in plastid-to-nucleus retrograde signaling in plants to ensure that nuclear gene expression is attuned to the needs of the chloroplast. Well-known sites of synthesis of chlorophyll for photosynthesis, plant chloroplasts also export heme and heme-derived linear tetrapyrroles (bilins), two critical metabolites respectively  ...[more]

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