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Î±-catulin play a role in lung cancer metastasis

ABSTRACT: Background: Î±-catulin may functions as an oncoprotein, sustaining proliferation by preventing cellular senescence and promoting cancer cell migration. In this study, we investigated the mechanism of Î±-catulin in cancer cell migration and metastasis in lung cancer. Method: Î±-catulin mRNA expression was isolated from A549/AS2neo (control) and A549/AS2neo-Î±-catulin stable cells. The Phalanx Human OneArray microarray analysis was performed to identify Î±-catulin downstream genes. Results: Overexpression of Î±-catulin increased cancer cell migration and metastasis. By using Phalanx Human OneArray microarray we have identified panel of genes altered by Î±-catulin overexpression, consisting of CDC42, intergrins and genes related to cytoskeleton remodeling. Conclusion: Î±-catulin is an oncoprotein and promotes lung cancer cell migration and metastasis. Two-condition experiment, Vector vs. alpha-catulin overexpression cells.The cDNAs encoding full-length human alpha-catulin were amplified and subcloned into lentiviral pLKO_AS2.neo which generated full-length alpha-catulin. Vector control or alpha-catulin lentivirus were transduced into A549 cells and G418 was used to select stable cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Tse-Ming Hong

PROVIDER: E-GEOD-40141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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