Unknown,Transcriptomics,Genomics,Proteomics

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Selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine.

ABSTRACT: The purpose of this study was to quantify the effects of basal leaf removal applied in Sangiovese cultivar at two different phonological stages, pre-bloom and veraison, on berry composition. As very few papers were published about the regulation of gene expression induced by vineyard management techniques, we report the first global transcriptomic analysis (integrated with agronomic and biochemical data) aiming to determine the molecular mechanisms that control Sangiovese berry composition. The comparison of gene expression profiles of defoliated vines at pre-bloom and at veraison with the control, revealed a common transcriptional response at the end of veraison in both treated berries, but also a more extensive transcriptome rearrangement in pre-bloom defoliated ones, which could be linked to the strong biochemical changes detected in the berry after pre-bloom veraison. Total RNA recovered from a pool of berries derived from three control plants (C) was compared to total RNA from a pool of berries derived from three defoliated vines at pre-bloom (PB, JD 147,6 basal leaves and relative laterals removed from each shoot) and three defoliated vines at veraison (V, JD 211,6 basal leaves and relative laterals removed from each shoot) at beginning of veraison (BV), end of veraison (EV) and harvest (H). For each sampling date microarray analyses were conducted for three different biological replicates for treatment.

ORGANISM(S): Vitis vinifera

SUBMITTER: Chiara Pastore

PROVIDER: E-GEOD-40487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine.

Pastore Chiara C Zenoni Sara S Fasoli Marianna M Pezzotti Mario M Tornielli Giovanni Battista GB Filippetti Ilaria I

BMC plant biology 20130222

<h4>Background</h4>The selective removal of grapevine leaves around berry clusters can improve the quality of ripening fruits by influencing parameters such as the berry sugar and anthocyanin content at harvest. The outcome depends strongly on the timing of defoliation, which influences the source-sink balance and the modified microclimate surrounding the berries. We removed the basal leaves from Vitis vinifera L. cv Sangiovese shoots at the pre-bloom and veraison stages, and investigated respon ...[more]

PMID: 23433030

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Dataset Information

Selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine.

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Selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine.

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