Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling of soybean genes in response to drought (reproductive stage)

ABSTRACT: Drought-responsive genes in soybean leaves were successfully identified using Affymetrix Soybean Gene 1.0 ST arrays on leaves samples of reproductive-stage soybean plants. R1 soybean plants planted in pots were imposed drought by withholding water for 5 days until the soil moisture content dropped to 5%, and 3rd trifoliates (now at the R2 stage) were collected for expression profiling. Soybean plants were grown in pots. When the plants reached the R1 stage (started flowering), drought treatment was imposed by withholding water. The soil moisture content was monitored during the process until the 5th day of water withholding, when soil moisture content reached 5%. The 3rd trifoliate (counting from shoots), now at the R2 stage, was collected for total RNA extraction, while other 3rd trifoliates of similar chlorophyl index were collected for leaves water content determination to identify the severity of the stress. Total RNA from 3rd trifoliates were used for expression profiling using Affymetrix Soybean Gene 1.0 ST arrays. Four biological repeats per treatment were performed, three biological repeats were chosen for expression profiling.

ORGANISM(S): Glycine max

SUBMITTER: Dung Le

PROVIDER: E-GEOD-40604 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression profiling of soybean genes in response to drought

Project description:Drought-responsive genes in soybean leaves were successfully obtained using soybean gene 1.0 ST array. Leaf samples from the vegetative stage of soybean plants were used. V6 soybean plants planted in the pots were imposed drought by withholding water for 6 days until the soil moisture content drop to 5% and trifolium 4th were collected for expression profiling

2012-04-02 | E-GEOD-29663 | biostudies-arrayexpress

Expression profiling of soybean genes in response to drought (reproductive stage)

Project description:Drought-responsive genes in soybean leaves were successfully identified using Affymetrix Soybean Gene 1.0 ST arrays on leaves samples of reproductive-stage soybean plants. R1 soybean plants planted in pots were imposed drought by withholding water for 5 days until the soil moisture content dropped to 5%, and 3rd trifoliates (now at the R2 stage) were collected for expression profiling.

2012-11-10 | GSE40604 | GEO

Expression profiling of soybean genes in response to drought

Project description:This SuperSeries is composed of the following subset Series: GSE29663: Expression profiling of soybean genes in response to drought (vegetative stage) GSE40604: Expression profiling of soybean genes in response to drought (reproductive stage) Refer to individual Series

2012-11-10 | E-GEOD-40627 | biostudies-arrayexpress

Expression data from Arabidopsis flowers under moderate drought stress

Project description:We also used microarray analysis to examine transcriptomic changes under moderate drought, identifying thousends of genes that potentially mediate moderate drought responses in the flower, including genes encoding transcription factors that likely play crucial regulatory roles. Arabidopsis were well-watered until after just bolting (after 24 days growth with the main stem about 1 cm high) when moderate drought treatment was started by withholding water (defined as day 0 for moderate drought treatment). The relative soil moisture content decreased rapidly and, after about 48 hours the relative soil moisture content was near 50% of the soil water-holding capacity (first moderate drough treated sample M3 were collected at day 3 (72 h after withholding water)). The soil water condition was maintained by daily watering until almost all the fruits were mature and ready to harvest (about 50 days). For well-watered (control) plants, 90% of the soil water-holding capacity was maintained until tissue harvest or after seed maturation (pots were weighed and watered twice per day). Unopened floral bud samples were collected at day 0, 3, 4, 5, 10.

2014-06-01 | E-GEOD-55431 | biostudies-arrayexpress

dreb1a expression data from Arabidopsis flowers under drought stress

Project description:We used microarray analysis to examine transcriptomic changes upon dreb1a under drought, identifying hundreds of genes that potentially function downstream of DREB1A and mediate drought responses in the flower, including genes encoding transcription factors that likely play crucial regulatory roles. DREB1a mutant (CS872453) were well-watered until after just bolting (after 24 days growth with the main stem about 1 cm high) when drought treatment was started by withholding water (defined as day 0 for drought treatment). The relative soil moisture content decreased sharply and, after about 80 hours (defined as day 3 for drought treatment), the relative soil moisture content was near 35% of the soil water-holding capacity. The soil water condition was maintained by daily watering until almost all the fruits were mature and ready to harvest. Unopened flower samples were collected from drought treated plants, at day 3, 4, 5.

2013-11-06 | E-GEOD-43724 | biostudies-arrayexpress

Expression data from Arabidopsis flowers under drought stress

Project description:We also used microarray analysis to examine transcriptomic changes under drought, identifying thousands of genes that potentially mediate drought responses in the flower, including genes encoding transcription factors that likely play crucial regulatory roles. Arabidopsis were well-watered until after just bolting (after 24 days growth with the main stem about 1 cm high) when drought treatment was started by withholding water (defined as day 0 for drought treatment). The relative soil moisture content decreased sharply and, after about 80 hours (defined as day 3 for drought treatment), the relative soil moisture content was near 35% of the soil water-holding capacity. The soil water condition was maintained by daily watering until almost all the fruits were mature and ready to harvest (about 50 days). For well-watered (control) plants, 90% of the soil water-holding capacity was maintained until tissue harvest or after seed maturation (pots were weighed and watered twice per day). Unopened flower samples were collected, from both treated and control plants, at day 0, 3, 4, 5, 10.

2013-11-06 | E-GEOD-40998 | biostudies-arrayexpress

Molecular dissection of drought tolerance mechanisms in rice by comparative deep transcriptome sequencing

Project description:To dissect the molecular mechanisms underlying drought tolerance (DT) in rice, transcriptome differences of a DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing. Results revealed a differential constitutive gene expression prior to stress and distinct global transcriptome reprogramming among three genotypes under time-series drought stress, consistent with their differential genotypes and DT phenotypes. DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing.The drought stress treatment was started by withholding water at the tillering stage. The days were counted after the AWC in the soil reached 20% to allow drought measurements at precisely determined intervals, and the soil water content reached 15%, 10% and 7.5% after 1d, 3d and 4d drought treatment, respectively.Three top leaves for each sample were harvested for each genotype under 1d and 3d drought stress and control conditions. All samples were immediately frozen in liquid nitrogen and stored at -80C and then for transcriptome sequencing.

2014-12-30 | E-GEOD-57950 | biostudies-arrayexpress

Transcriptomics and physiological analyses reveal coordinated alteration of metabolic pathways in Jatropha curcas drought tolerance

Project description:Jatropha curcas, a multipurpose plant attracting much attention due to its high oil content and quality for biofuel, is recognized as a drought tolerant species. However, this drought tolerance is still poorly characterized. This study aims to contribute to uncover the molecular background of this tolerance, with the use of a combined approach of transcriptional profiling and morphophysiological characterization along a period of water withholding (49 days) followed by rewatering (7 days). Morphophysiological measurements evidenced that J. curcas plants presented different adaptations to withstand moderate and severe drought. Thus, RNA-Seq was performed for samples collected at moderate and severe stress followed by rewatering, for both roots and leaves. Transcriptomic analysis revealed organ-specific adaptations across all investigated conditions, except under severe stress, in which the drought response of J. curcas surpassed organ-specificity by dramatic transcriptomic reorganization. These changes in gene expression were clearly evidenced by the down-regulation of genes involved in growth and water uptake, and up-regulation of osmotic adjustments and cellular homeostasis related genes. However, organ-specific variations were also detected, such as strong up-regulation of chlorophyll and trehalose metabolism in leaves. Functional validation further corroborated the differentially expression of genes coding for enzymes involved in chlorophyll metabolism, which correlates with the metabolite content of this pathway. Two Jatropha curcas accessions were submitted to moderate and severe drought stress (water withholding) followed by recovery (3d re-watering), transcriptomic profiles were assessed by RNA-Seq.

2015-12-09 | E-GEOD-61109 | biostudies-arrayexpress

Comparative expression analysis of roots of two soybean cultivars with contrasting drought-tolerant phenotype under well-watered and dehydration conditions.

Project description:To understand the responses of plants to environmental stresses will help mitigate the problems via creating stress-tolerant crop cultivars. We have carried out comparative expression analysis of roots of two soybean varieties Williams 82 and DT2008 that have constrasting drought-responsive phenotype under dehydration and well-watered (control) conditions. Affymetrix’s whole Soybean Gene Expression Microarray (66K) was used. The Williams 82 and DT2008 soybean plants were grown for 14 days in the vermiculite soil under greenhouse conditions. The whole plants of 14-d-old plants were detached and exposed to dehydration on KimTowel papers for 0 (well-watered, control), 2 and 10 h. All roots of independent 14-d-old plants were collected. Total RNA was prepared and used for the microarray hybridization. Three independent biological replicates were used for each plant sample.

2015-07-02 | E-GEOD-65553 | biostudies-arrayexpress

Expression data from Soybean leaves under drought stress and normally irrigated

Project description:BiP overexpression confers resistance to drought, through an as yet unknown mechanism that is related to ER functioning, has been described that the delay in leaf senescence by BiP overexpression might relate to the absence of the response to drought. We used microarrays to detail the global programme of gene expression underlying in soybean leaves overexpressing BiP and with different water potentials to elucidate the mechanisms by which BiP protects plant cells against cell death induced by dehydration. BiP overexpressing plants and wild type as a control were subjected to dry slowly for 25 days. Third or fourth trifoliate leaves were collected from plants in water potential next 1.0 MPa, 1.5 MPa and 1.7 MPa for test weak and strong stress and subject RNA extraction and hybridization on Affymetrix microarrays maintaining the hybridization of two chips per treatment.

2013-08-29 | E-GEOD-50408 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data