DNA methylation in CD14 and CD34 blood cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Timothy Myers
PROVIDER: E-GEOD-40790 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34+ cells.
Merling Randall K RK Sweeney Colin L CL Choi Uimook U De Ravin Suk See SS Myers Timothy G TG Otaizo-Carrasquero Francisco F Pan Jason J Linton Gilda G Chen Lifeng L Koontz Sherry S Theobald Narda L NL Malech Harry L HL
Blood 20130205 14
A variety of somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs), but CD34(+) hematopoietic stem cells (HSCs) present in nonmobilized peripheral blood (PB) would be a convenient target. We report a method for deriving iPSC from PB HSCs using immunobead purification and 2- to 4-day culture to enrich CD34(+) HSCs to 80% ± 9%, followed by reprogramming with loxP-flanked polycistronic (human Oct4, Klf4, Sox2, and c-Myc) STEMCCA-loxP lentivector, or with Sendai vectors. Coloni ...[more]
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