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Transcriptome-wide analyses of CstF64-RNA interactions in global regulation of mRNA alternative polyadenylation

ABSTRACT: We mapped CstF64-RNA interactions at the transcriptome level and studied CstF64-mediated regulation of global mRNA alternative polyadenylation by using iCLIP-seq and direct RNA sequencing analyses. CstF64 iCLIP-seq in HeLa cells; direct RNA sequencing (DRS) of control HeLa cells, CstF64-RNAi cells and CstF64&CstF64tau-RNAi cells

ORGANISM(S): Homo sapiens

SUBMITTER: Yongsheng Shi

PROVIDER: E-GEOD-40859 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Our study of CstF64 and CstF64τ revealed both functional overlap and specificity of these two important mRNA 3’ processing factors and provided new insights into the regulatory mechanisms of mRNA 3’ processing. CstF64tau iCLIP-seq in HeLa cells; direct RNA sequencing (DRS) of control HeLa cells, CstF64tau-RNAi cells and SELEX-seq for RNA control pool, CstF64 and CstF64tau.

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