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Gene expression profiles induced by overexpression of PDEF in MCF10A mammary epithelial cell line

ABSTRACT: Recent studies suggest that PDEF is required for secretory cell differentiation in several epithelial tissues. To investigate PDEF in the mammary gland, we examined the effect of this transcription factor on gene expression using microarray based profiling of MCF-10A cells. These cells are non-transformed mammary epithelial cells that express protein and gene expression programs of basal epithelial cells and undetectable levels of endogenous PDEF. Bioinformatics analysis of the genes induced or repressed by PDEF overexpression in MCF10A cells revealed a striking effect on expression of luminal and myoepithelial cell markers. Six samples were harvested 26 hours after retroviral infection with either vector control or PDEF. Each condition was performed in triplicate.

ORGANISM(S): Homo sapiens

SUBMITTER: Joan Brugge

PROVIDER: E-GEOD-40986 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PDEF promotes luminal differentiation and acts as a survival factor for ER-positive breast cancer cells.

Buchwalter Gilles G Hickey Michele M MM Cromer Anne A Selfors Laura M LM Gunawardane Ruwanthi N RN Frishman Jason J Jeselsohn Rinath R Lim Elgene E Chi David D Fu Xiaoyong X Schiff Rachel R Brown Myles M Brugge Joan S JS

Cancer cell 20130601 6

Breast cancer is a heterogeneous disease and can be classified based on gene expression profiles that reflect distinct epithelial subtypes. We identify prostate-derived ETS factor (PDEF) as a mediator of mammary luminal epithelial lineage-specific gene expression and as a factor required for tumorigenesis in a subset of breast cancers. PDEF levels strongly correlate with estrogen receptor (ER)-positive luminal breast cancer, and PDEF transcription is inversely regulated by ER and GATA3. Furtherm ...[more]

PMID: 23764000

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Gene expression profiles induced by overexpression of PDEF in MCF10A mammary epithelial cell line

Project description:Recent studies suggest that PDEF is required for secretory cell differentiation in several epithelial tissues. To investigate PDEF in the mammary gland, we examined the effect of this transcription factor on gene expression using microarray based profiling of MCF-10A cells. These cells are non-transformed mammary epithelial cells that express protein and gene expression programs of basal epithelial cells and undetectable levels of endogenous PDEF. Bioinformatics analysis of the genes induced or repressed by PDEF overexpression in MCF10A cells revealed a striking effect on expression of luminal and myoepithelial cell markers.

2014-03-29 | GSE40986 | GEO

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Global gene expresion profile of phenotypically distinct human mammary epithelial subpopulations of various developmental age

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Expression data of pre-stasis cultured HMEC

Project description:We have generated a large collection of normal human mammary epithelial cell strains from women aged 16 to 91 years, derived from primary tissues, to enable functional and molecular interrogation of aging. We demonstrate in finite-lifespan cultured and uncultured epithelial cells that aging is associated with reduction of myoepithelial cells and with increases in luminal cells expressing keratin 14 and integrin Î±6, traits that are expressed exclusively in myoepithelial cells in women under 30. We find that changes to the luminal lineage result from age-dependent expansion of multipotent progenitors that bear defects resulting in incompletely differentiated luminal cells. These findings were verified in vivo in normal breast tissues. Myoepithelial cells have been suggested to act as tumor suppressors, and progenitor cells are implicated as the etiological roots of mammary carcinomas. Thus with aging there is a shift in the balance of luminal/myoepithelial lineages, and changes in the functional spectrum of multipotent progenitors, which presages increased potential for malignant transformation. Finite lifespan pre-stasis HMEC from specimens from reduction mammoplasties of 7 patients of different age were collected. The expression data was queried for different biological replicates and at different passages. For strain 240, luminal and myoepithelial cells were collected using flow cytometry.

2012-05-02 | E-GEOD-37485 | biostudies-arrayexpress

14-3-3ζ overexpression-induced gene signature in MCF-10A cells

2013-11-05 | GSE52032 | GEO

Expression profiling of MCF10A cells in 2D and 3D culture

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2011-06-01 | E-GEOD-26148 | biostudies-arrayexpress

14-3-3ζ overexpression-induced gene signature in MCF-10A cells

Project description:To study the function of 14-3-3ζ, we established MCF-10A human mammary epithelial cells transduced with 14-3-3ζ (10A.ζ) and vector (10A.Vec) We performed gene expression profiling on 10A.ζ cells and 10A.Vec cells, and normalized to profiling of MCF-10A parental cells Total mRNA were extracted from 10A.Parental cells, 10A.Vec cells, 10A.ζ cells which were cultured in 3D culture model for 16 days, and subjected to Affymetrix microarray analysis

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Expression data of pre-stasis cultured HMEC

Project description:We have generated a large collection of normal human mammary epithelial cell strains from women aged 16 to 91 years, derived from primary tissues, to enable functional and molecular interrogation of aging. We demonstrate in finite-lifespan cultured and uncultured epithelial cells that aging is associated with reduction of myoepithelial cells and with increases in luminal cells expressing keratin 14 and integrin α6, traits that are expressed exclusively in myoepithelial cells in women under 30. We find that changes to the luminal lineage result from age-dependent expansion of multipotent progenitors that bear defects resulting in incompletely differentiated luminal cells. These findings were verified in vivo in normal breast tissues. Myoepithelial cells have been suggested to act as tumor suppressors, and progenitor cells are implicated as the etiological roots of mammary carcinomas. Thus with aging there is a shift in the balance of luminal/myoepithelial lineages, and changes in the functional spectrum of multipotent progenitors, which presages increased potential for malignant transformation.

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