Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcriptome analysis of Dunaliella viridis in response to changes in light duration and temperature


ABSTRACT: Transcriptome analysis of Dunaliella viridis in response to changes in light duration and temperature Time course experiment experiment with 5 evenly spaced time points, t1-t5, and two experimental factors: a) light condition (2 levels: continuous light and light/dark cycle) and b) temperature (2 levels: 25 degrees Celsius and 35 degrees Celsius). Cells grown in the light/dark condition were exposed to a 12 hour light/dark cycle, whereas continuous light samples were grown under constant light conditions. All cells were grown at 25 degrees Celsius for timepoints t1 and t2; at time t3, cells grown in the high temperature condition were raised to 35°C, and kept at this temperature for the remainder of the experiment. Bar-coded samples were sequenced in two separate sequencing runs using 100-nt Illumina RNA-Seq reads. In the first run a single pooled biological replicate was sequenced from each of the 12 combinations of time, light condition and temperature from time points t3 to t5. The bar-coded samples were divided among 4 Illumina Lanes resulting in 48 distinct sequencing data files. In the second run ("Run 2") a single bar-coded biological replicate was sequenced from each of the 10 combinations of time and light condition from time points t1 through t5, with temperature fixed at 25°C.

ORGANISM(S): Dunaliella viridis

SUBMITTER: Brian Howard 

PROVIDER: E-GEOD-40997 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2019-04-17 | GSE116123 | GEO
| PRJNA244106 | ENA
2024-02-16 | PXD037343 | Pride
2023-09-04 | PXD029114 | Pride
2022-10-23 | GSE159988 | GEO
2021-10-26 | GSE178750 | GEO
2024-01-01 | GSE224529 | GEO
2009-12-08 | E-GEOD-9495 | biostudies-arrayexpress
2022-02-15 | PXD014765 | Pride
2023-03-11 | PXD032754 | Pride