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Transcription profiling of human fibroblast IMR-90 cells tissue culture polystyrene time series

ABSTRACT: IMR-90 Human Fibroblasts were seeded onto TCPS dishes. mRNA was isolated at 1, 2, 4, 8, 12, 24, 48 and 96 hours. This time course was used as a control for gene expression studies on collagen/chondroitin sulfate tissue engineering scaffold materials using the same cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Catherine Klapperich

PROVIDER: E-GEOD-410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global gene expression of cells attached to a tissue engineering scaffold.

Klapperich Catherine M CM Bertozzi Carolyn R CR

Biomaterials 20041101 25

A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold mate ...[more]

PMID: 15159079

Similar Datasets

IMR-90 cells: Tissue Culture Polystyrene Time Course

Project description:IMR-90 Human Fibroblasts were seeded onto TCPS dishes. mRNA was isolated at 1, 2, 4, 8, 12, 24, 48 and 96 hours. This time course was used as a control for gene expression studies on collagen/chondroitin sulfate tissue engineering scaffold materials using the same cell line. Keywords = tissue culture plates Keywords: time-course

2003-09-10 | GSE410 | GEO

IMR-90 cells: Collagen Mesh Time Course

Project description:A series of chips with some repeated measurments. Each chip represents a pool of 4 collagen/chondroitin sulfate tissue engineering scaffold meshes seeded with 1 x 10^6 IMR-90 Human Fibroblasts. mRNA was isolated at 30 minutes, 1, 2, 4, 8, 12, 24, 48, and 72 hours after seeding. Keywords = tissue engineering mesh, collagen, chondroitin sulfate, biomaterials design Keywords: time-course

2003-09-10 | GSE409 | GEO

Transcription profiling time series of the growth of human IMR-90 cells seeded on a collagen/chondroitin sulfate tissue engineering scaffold

2007-12-13 | E-GEOD-409 | biostudies-arrayexpress

miR-29 targets in human fetal lung fibroblast IMR-90 cells

Project description:TGFβ is one of most intensively studied regulators of extracellular matrix formation, and has been implicated in the development of pulmonary fibrosis in different models. However, little is know about the role of miRNAs in TGFβ mediated fibrogenic gene regulation. By using miRNA qRT-PCR array, we have identified miRNAs whose expression are regulated by TGFβ in IMR-90 cells. Among those down-regulated miRNAs are miR-29 family members. Knockdown miR-29 in IMR-90 cells results in up-regulation of a large number of extracellular matrix and fibrogenic genes including family members of collagen, laminin, integrin, ADAM and MMP, many of them are predicted or confirmed miR-29 targets. Hierarchichal clustering analysis of mRNA array data revealed that many extracellular matrix and fibrogenic genes up-regulated by TGFβ in IMR-90 cells, are also up-regulated in miR-29 KD cells. Moreover, the similar set of extracellular matrix and fibrogenic genes is also significantly up-regulated in bleomycin treated mouse lungs. Together, our data strongly suggest that downstream of the TGFβ, miR-29 is a master modulator of genes involved in extracellular matrix formation and might play a significant role in pulmonary fibrosis.

2010-03-01 | GSE18651 | GEO

ChIP-seq from IMR-90 (ENCSR970FKL)

Project description:ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE92197 | GEO

ChIP-seq from IMR-90 (ENCSR664OKA)

Project description:ELK1 ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE91969 | GEO

ChIP-seq from IMR-90 (ENCSR124AIG)

Project description:FOS ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-10-20 | GSE105300 | GEO

microRNA-seq from IMR-90 (ENCSR123QKC)

Project description:RNA Evaluation IMR-90 microRNA-seq from Mortazavi For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-06-15 | GSE176683 | GEO

intact Hi-C from IMR-90 (ENCSR345VTI)

Project description:IMR-90 intact Hi-C For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2023-07-24 | GSE237347 | GEO

Sequencing of human IMR-90 at 27 and 55 population doublings

Project description:Comparisong of gene expression of human IMR-90 at 27 and 55 population doublings. RNA-seq data comprises 2 groups: 27 and 55 population doublings of IMR-90. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

2021-10-19 | GSE117813 | GEO

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