Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data between FOXP3 wild type and 2T>C(mut)

ABSTRACT: Investigation of the role of FOXP3 in CD4+ T effector cells. FOXP3 is transiently upregulated in T effector cells under activation. This temporary expression in Teff cells is insufficient to suppress expression of reported targets of FOXP3 repressor activity. The role of FOXP3 in T effector cells remains unclear. We used microarray analysis to detail the differentially expressed genes between FOXP3 wild type and 2T>C(mut) clones and identified classes of up-regulated or down-regulated genes based upon FOXP3 expression. We used T effector cells from one IPEX disease carrier mother that consist of a mixed population ofFOXP3 wild type and 2T>C(mut) clones. We activated them using anti-CD3, anti-CD28. We compareFOXP3 wild type and 2T>Cl clones at different stages: resting phase and activated phase at 72hrs.

ORGANISM(S): Homo sapiens

SUBMITTER: Jose Garcia-Manteiga

PROVIDER: E-GEOD-41087 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A novel function for FOXP3 in humans: intrinsic regulation of conventional T cells.

McMurchy Alicia N AN Gillies Jana J Gizzi Maria Concetta MC Riba Michela M Garcia-Manteiga Jose Manuel JM Cittaro Davide D Lazarevic Dejan D Di Nunzio Sara S Piras Ignazio S IS Bulfone Alessandro A Roncarolo Maria Grazia MG Stupka Elia E Bacchetta Rosa R Levings Megan K MK

Blood 20121120 8

The role of forkhead box P3 (FOXP3) is well-established in T-regulatory cells, but the function of transient FOXP3 expression in activated human conventional T (Tconv) cells is unknown. In the present study, we used 2 approaches to determine the role of FOXP3 in human Tconv cells. First, we obtained Tconv clones from a female subject who is hemizygous for a null mutation in FOXP3, allowing the comparison of autologous T-cell clones that do or do not express FOXP3. Second, we knocked down activat ...[more]

PMID: 23169781

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Dataset Information

Expression data between FOXP3 wild type and 2T>C(mut)

Publications

A novel function for FOXP3 in humans: intrinsic regulation of conventional T cells.

Similar Datasets

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