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Genes differentially expressed in Haloferax mediterranei strains, DF50 and M-NM-^TdeoR2, with or without induction by fructose

ABSTRACT: Transcriptional profiling of Haloferax mediterranei DF50 and M-NM-^TdeoR2 with induction by fructose comparing with the strains without this induction. Goal was to explore the effect of induction by fructose on Haloferax mediterranei. Total RNA from the Haloferax mediterranei DF50 and M-NM-^TdeoR2 with or without induction by fructose were used to generate target cDNA, and then hybridized to Haloferax mediterranei genome array genechips, representing about 3800 genes.

ORGANISM(S): Haloferax mediterranei

SUBMITTER: Lei Cai 

PROVIDER: E-GEOD-41134 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of the transcriptional regulator GlpR, promoter elements, and posttranscriptional processing involved in fructose-induced activation of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Haloferax mediterranei.

Cai Lei L   Cai Shuangfeng S   Zhao Dahe D   Wu Jinhua J   Wang Lei L   Liu Xiaoqing X   Li Ming M   Hou Jing J   Zhou Jian J   Liu Jingfang J   Han Jing J   Xiang Hua H  

Applied and environmental microbiology 20131213 4

Among all known archaeal strains, the phosphoenolpyruvate-dependent phosphotransferase system (PTS) for fructose utilization is used primarily by haloarchaea, which thrive in hypersaline environments, whereas the molecular details of the regulation of the archaeal PTS under fructose induction remain unclear. In this study, we present a comprehensive examination of the regulatory mechanism of the fructose PTS in the haloarchaeon Haloferax mediterranei. With gene knockout and complementation, micr  ...[more]

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