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OsJMJ703, a Histone H3K4 Demethylase, Controls Transposon Activity in Rice


ABSTRACT: Retrotransposons are classified into long terminal repeat (LTR) or non-LTR retrotransoposons, and both types can mobilize in a “copy and paste” manner. Rice genome contains >40% of repetitive sequences or transposable elements (TEs) that scattered throughout the genome. TE activities are tightly regulated by distinct epigenetic mechanisms. Histone lysine methylation is catalyzed by SET domain group (SDG) protein and reversed by a family of Jumonji C (JmjC) domain-containing proteins. In rice, DNA methylation and histone methylation have been implicated in controlling copia-like LTR retrotransposon Tos17 activities. Whether any TEs are controlled by histone demethylase remains to be elusive. Here, we show that JMJ703 is a histone H3K4 specific demethylase in rice. Impaired JMJ703 disrupted genome-wide transcriptome and enhanced H3K4me3 resulting in pleiotropic defects. Two LINE elements, Karma and its N-terminal truncation were identified as direct targets of JMJ703 with increased frequency of transposition in jmj703, while Tos17 is unaffected. Our findings show that plants use H3K4me3 demethylase to constitutively remove active chromatin mark and maintain silencing status at a subset retrotransposons which localize in heterochromatin regions. Therefore, our work uncovers a novel mechanism to control retrotransposon activity by histone demethylation, which further strengthens the link between epigenetic silencing and genome stability. Examination of differences in H3K4 between jmj703 and wild type.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: xiaofeng cao 

PROVIDER: E-GEOD-41183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Control of transposon activity by a histone H3K4 demethylase in rice.

Cui Xiekui X   Jin Ping P   Cui Xia X   Gu Lianfeng L   Lu Zhike Z   Xue Yongming Y   Wei Liya L   Qi Jianfei J   Song Xianwei X   Luo Ming M   An Gynheung G   Cao Xiaofeng X  

Proceedings of the National Academy of Sciences of the United States of America 20130114 5


Transposable elements (TEs) are ubiquitously present in plant genomes and often account for significant fractions of the nuclear DNA. For example, roughly 40% of the rice genome consists of TEs, many of which are retrotransposons, including 14% LTR- and ∼1% non-LTR retrotransposons. Despite their wide distribution and abundance, very few TEs have been found to be transpositional, indicating that TE activities may be tightly controlled by the host genome to minimize the potentially mutagenic effe  ...[more]

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