Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111


ABSTRACT: Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production. Burkholderia cenocepacia BDSF stimulon was determined in complex media using Illumina RNA-Seq

ORGANISM(S): Burkholderia cenocepacia H111

SUBMITTER: Gabriella Pessi 

PROVIDER: E-GEOD-41244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111.

Schmid Nadine N   Pessi Gabriella G   Deng Yinyue Y   Aguilar Claudio C   Carlier Aurelien L AL   Grunau Alexander A   Omasits Ulrich U   Zhang Lian-Hui LH   Ahrens Christian H CH   Eberl Leo L  

PloS one 20121120 11


Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the ide  ...[more]

Similar Datasets

2012-11-01 | GSE41244 | GEO
2013-07-15 | E-GEOD-48585 | biostudies-arrayexpress
2014-03-26 | E-GEOD-52769 | biostudies-arrayexpress
2011-05-01 | E-MTAB-509 | biostudies-arrayexpress
2014-03-26 | GSE52769 | GEO
2015-04-29 | GSE66328 | GEO
| PRJNA69823 | ENA
2013-07-15 | GSE48585 | GEO
2015-04-29 | E-GEOD-66328 | biostudies-arrayexpress
2015-05-05 | PXD001924 | Pride