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Exploring the larval transcriptome of the common sole (Solea solea L.)


ABSTRACT: A microarray platform for Solea solea was developed to study gene expression profiles of common sole developmental stages. Characterization of the transcriptome of S. solea, focusing on larval and juvenile stages. After transcriptome sequencing and annotation [TSA accession number GAAQ00000000], an oligo-DNA microarray for the detection of 12,836 unique transcripts was developed and applied to the study of gene expression profiles during larval-to-juvenile transition. For microarray experiments, pooled samples of 10–5 individuals (depending on larval age) were sampled and RNA extracted at 1, 4, 6, 11, 13, 18, 24 and 33 days post-hatching (dph). The number of biological replicates was four for each sampling point.

ORGANISM(S): Solea solea

SUBMITTER: Serena Ferraresso 

PROVIDER: E-GEOD-41261 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exploring the larval transcriptome of the common sole (Solea solea L.).

Ferraresso Serena S   Bonaldo Alessio A   Parma Luca L   Cinotti Stefano S   Massi Paola P   Bargelloni Luca L   Gatta Pier Paolo PP  

BMC genomics 20130510


<h4>Background</h4>The common sole (Solea solea) is a promising candidate for European aquaculture; however, the limited knowledge of the physiological mechanisms underlying larval development in this species has hampered the establishment of successful flatfish aquaculture. Although the fact that genomic tools and resources are available for some flatfish species, common sole genomics remains a mostly unexplored field. Here, we report, for the first time, the sequencing and characterisation of  ...[more]

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