Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

24 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from follicular lymphoma cells cultured either in suspension either as Multicellular aggregates of lymphoma cells (MALC)

ABSTRACT: Follicular Lymphomas are blood tumors growing as spheres in patients. Before this study, there was no experimental model mimicking the 3D organization of these in vivo tumors. We develop such a model, called MALC, and performed a pan-genomic comparative analysis between MALC and classical suspension cultures. We used microarrays to detail the global gene expression profile induced by aggregated growth of lymphoma cells. 10 days-old MALC and suspension RL cells were selected for RNA extraction and hybridization on Affymetrix U133+ 2.0 microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Pauline GRAVELLE

PROVIDER: E-GEOD-41851 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression data from follicular lymphoma cells cultured either in suspension either as Multicellular aggregates of lymphoma cells (MALC)

2012-10-27 | GSE41851 | GEO

Expression data from quiescent cells and cycling cells isolated from Multicellular aggregates of lymphoma cells (MALC)

Project description:Follicular Lymphomas are blood tumors growing as spheres in patients. Before this study, there was no experimental model mimicking the 3D organization of these in vivo tumors. We develop such a model, called MALC, and observed a progressive enrichment in quiescent cells in these with time of culture; these cells were sorted, as their cycling counterparts, and their transcriptomes were compared. We used microarrays to detail the differential global gene expression profile between quiescent and cycling cells isolated from MALC. 14 days-old MALC were stained with pyronin Y and quiescent and cycling subpopulations sorted were selected for RNA extraction and hybridization on Affymetrix U133+ 2.0 microarrays.

2012-10-27 | E-GEOD-41855 | biostudies-arrayexpress

Cell growth in aggregates determines gene expression, proliferation, survival and chemoresistance of Follicular Lymphoma

Project description:This SuperSeries is composed of the following subset Series: GSE41851: Expression data from follicular lymphoma cells cultured either in suspension either as Multicellular aggregates of lymphoma cells (MALC) GSE41855: Expression data from quiescent cells and cycling cells isolated from Multicellular aggregates of lymphoma cells (MALC) Refer to individual Series

2012-10-27 | E-GEOD-41856 | biostudies-arrayexpress

Expression data from quiescent cells and cycling cells isolated from Multicellular aggregates of lymphoma cells (MALC)

2012-10-27 | GSE41855 | GEO

Differences in gene expression between multicellular spheres and adherent cell of tongue cancer cell lines CAL27 and HSC3

Project description:To investigate the differences in gene expression between multicellular spheres and adherent cell of tongue cancer cell, We compared the difference in gene expression between CAL27 and HSC3 cultured in suspension culture and adherent culture.

2024-05-12 | GSE228889 | GEO

Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates.

Project description:BackgroundHuman embryonic stem cells (hESC) should enable novel insights into early human development and provide a renewable source of cells for regenerative medicine. However, because the three-dimensional hESC aggregates [embryoid bodies (hEB)] typically employed to reveal hESC developmental potential are heterogeneous and exhibit disorganized differentiation, progress in hESC technology development has been hindered.Methodology/principal findingsUsing a centrifugal forced-aggregation strategy in combination with a novel centrifugal-extraction approach as a foundation, we demonstrated that hESC input composition and inductive environment could be manipulated to form large numbers of well-defined aggregates exhibiting multi-lineage differentiation and substantially improved self-organization from single-cell suspensions. These aggregates exhibited coordinated bi-domain structures including contiguous regions of extraembryonic endoderm- and epiblast-like tissue. A silicon wafer-based microfabrication technology was used to generate surfaces that permit the production of hundreds to thousands of hEB per cm(2).Conclusions/significanceThe mechanisms of early human embryogenesis are poorly understood. We report an ultra high throughput (UHTP) approach for generating spatially and temporally synchronised hEB. Aggregates generated in this manner exhibited aspects of peri-implantation tissue-level morphogenesis. These results should advance fundamental studies into early human developmental processes, enable high-throughput screening strategies to identify conditions that specify hESC-derived cells and tissues, and accelerate the pre-clinical evaluation of hESC-derived cells.

| S-EPMC2215775 | biostudies-literature

Hindlimb suspension and reloading study

Project description:Analysis of effect of hindlimb suspension and reloading on C57Bl/6 mouse soleus muscle. Experimental groups examined: -Control mice, 14 days -Hindlimb suspension for 7 days -Hindlimb suspension for 7 days, and subsequent reloading for 1 day -Hindlimb suspension for 7 days, and subsequent reloading for 7 days Keywords: other

2004-09-30 | GSE1293 | GEO

Environmental bacteria suspension

Project description:Environmental bacteria suspension Raw sequence reads

| PRJNA705060 | ENA

Biofilm and BES suspension

Project description:Biofilm and BES suspension Genome sequencing

| PRJNA686243 | ENA

Transcriptome profile in the human synovial MSC-aggregates

Project description:One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic potential of MSCs are needed to achieve successful cartilage regeneration by transplantation of a limited number of cells. Aggregated culture is a popular method in ES and iPS cells to maintain or enhance their potentials. Here we investigated gene expression profile of aggregated MSCs. 621 genes were up-regulated and 409 genes were down-regulated more than 5-fold in MSC-aggregates compared with the number in MSCs in a monolayer culture. The most up-regulated gene was BMP2, which is one of the genes involved in chondrogenesis. Anti-inflammatory genes were also up-regulated in MSC-aggregates. The microarray data for selected genes were confirmed by real-time PCR. Human synovial MSCs was isolated from synovium of 3 distinct donors. The gene expression profile of MSC-aggregates cultured in hanging drop for 3days was compared with that of MSCs in a monolayer culture.

2012-05-12 | E-GEOD-31980 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data