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Microarray profiling of total testicular microRNAs in mouse


ABSTRACT: Gene expression in mammalian testis undergoing spermatogenesis is under strict post transcriptional regulation and microRNAs are known to play a role in this regulation. Considering the time window of first wave of spermatogenesis, we did a microarray profiling of total testicular microRNAs in mouse and found several significant patterns of variable expression of these molecules during the different stages analyzed here. Mouse testis tissue were taken at three stages, 8 days, 16 days and 24 days, during first wave of spermatogenesis for RNA extraction and hybridization on Locked Nucleic Acid(LNA) technology based Exiqon microarray platform. We included two biological replicates per age group and comparisons were done between the samples with respect to their hybridization to the 'reference' sample which had all the six samples pooled together.

ORGANISM(S): Mus musculus

SUBMITTER: Pradeep Kumar 

PROVIDER: E-GEOD-41857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dramatic changes in 67 miRNAs during initiation of first wave of spermatogenesis in Mus musculus testis: global regulatory insights generated by miRNA-mRNA network analysis.

Sree Sreesha S   Radhakrishnan Karthika K   Indu Sivankutty S   Kumar Pradeep G PG  

Biology of reproduction 20140813 3


We mapped global changes in miRNA and mRNA profiles spanning the first wave of spermatogenesis using prepubertal (Postnatal Day 8 [P8]), pubertal (P16), and adolescent (P24) Mus musculus testes and identified the differential expression of 67 miRNAs and 8226 mRNAs. These two data sets were integrated into miRNA-dependent regulatory networks based on miRWalk predictions. In a network representing the P8 to P16 transition, downregulation of four miRNAs and upregulation of 19 miRNAs were linked wit  ...[more]

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