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Analysis of prebiotic carbohydrate utilization by Bifidobacterium lactis Bl-04 using differential transcriptomics

ABSTRACT: The global transcriptome of the Bifidobacterium animalis subsp. lactis Bl-04 was analyzed during exponential growth on 11 prebiotic carbohydrates and glucose to identify the specific gene cluster differentially upregulated in response to each carbohydrate. Affymetrix hybridization experiments were performed to compare the differential transcriptional profiles of the B. lactis Bl-04 at the early-log (OD600nm 0.3-0.5) phase. 12 carbohydrates were tested with two technical replicates to each condition for a total of 24 hybridizations.

ORGANISM(S): Bifidobacterium animalis subsp. lactis

SUBMITTER: Todd Klaenhammer

PROVIDER: E-GEOD-41906 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04.

Andersen Joakim M JM Barrangou Rodolphe R Abou Hachem Maher M Lahtinen Sampo J SJ Goh Yong Jun YJ Svensson Birte B Klaenhammer Todd R TR

BMC genomics 20130510

<h4>Background</h4>Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of α- ...[more]

PMID: 23663691

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Analysis of prebiotic carbohydrate utilization by Bifidobacterium lactis Bl-04 using differential transcriptomics

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Transcriptional and functional analysis of Bifidobacterium animalis subsp. lactis exposure to tetracycline

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Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

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Expression data from Caenorhabditis elegans fed with the bacterial strain Bifidobacterium animalis sbsp. lactis CECT 8145

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