Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Jian-Liang Li
PROVIDER: E-GEOD-41996 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Joo Jeong-Hoon JH Correia Greg P GP Li Jian-Liang JL Lopez Maria-Cecilia MC Baker Henry V HV Sugrue Stephen P SP
Investigative ophthalmology & visual science 20130123 1
<h4>Purpose</h4>We investigated the impact of PININ (PNN) and epithelial splicing regulatory protein 1 (ESRP1) on alternative pre-mRNA splicing in the corneal epithelial context.<h4>Methods</h4>Isoform-specific RT-PCR assays were performed on wild-type and Pnn knockout mouse cornea. Protein interactions were examined by deconvolution microscopy and co-immunoprecipitation. For genome-wide alternative splicing study, immortalized human corneal epithelial cells (HCET) harboring doxycycline-inducibl ...[more]