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Comparison of extravillous trophoblast cells derived from human embryonic stem cells and from first trimester human placentas

ABSTRACT: The goal of the present study was to characterize HLA-G-positive cells derived from human embryonic stem cells treated with BMP4 for five days and compare them to HLA-G positive cells derived from human first trimester placentas Human embryonic stem cells were cultured with BMP4 in the absence of FGF2 for 5 days. They were then dispersed and separated into HLA-G+ and HLA-G-, TACSTD2 positive cells by using immunomagnetic bead separation. First trimester human placental samples from 8-12 weeks gestation were enzymatically separated into single cells, and then also separated into HLA-G+ and HLA-G-/TACSTD2+ cell populations

ORGANISM(S): Homo sapiens

SUBMITTER: Laura Schulz

PROVIDER: E-GEOD-42112 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of extravillous trophoblast cells derived from human embryonic stem cells and from first trimester human placentas.

Telugu B P BP Adachi K K Schlitt J M JM Ezashi T T Schust D J DJ Roberts R M RM Schulz L C LC

Placenta 20130428 7

<h4>Introduction</h4>Preeclampsia and other placental pathologies are characterized by a lack of spiral artery remodeling associated with insufficient invasion by extravillous trophoblast cells (EVT). Because trophoblast invasion occurs in early pregnancy when access to human placental tissue is limited, there is a need for model systems for the study of trophoblast differentiation and invasion. Human embryonic stem cells (hESC) treated with BMP4- differentiate to trophoblast, and express HLA-G, ...[more]

PMID: 23631809

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