Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Laura Banaszynski
PROVIDER: E-GEOD-42153 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Banaszynski Laura A LA Wen Duancheng D Dewell Scott S Whitcomb Sarah J SJ Lin Mingyan M Diaz Nichole N Elsässer Simon J SJ Chapgier Ariane A Goldberg Aaron D AD Canaani Eli E Rafii Shahin S Zheng Deyou D Allis C David CD
Cell 20130901 1
Polycomb repressive complex 2 (PRC2) regulates gene expression during lineage specification through trimethylation of lysine 27 on histone H3 (H3K27me3). In Drosophila, polycomb binding sites are dynamic chromatin regions enriched with the histone variant H3.3. Here, we show that, in mouse embryonic stem cells (ESCs), H3.3 is required for proper establishment of H3K27me3 at the promoters of developmentally regulated genes. Upon H3.3 depletion, these promoters show reduced nucleosome turnover mea ...[more]