Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide gene expression analysis in Thoc5- and CFIm68-depleted HeLa cells


ABSTRACT: We found that CFIm68, a mRNA cleavage and polyadenylation factor implicated for alternative polyadenylation site choice, was co-purified with Thoc5, a component of human THO/TREX. Microarray analysis using human HeLa cells reveals knockdown of Thoc5 affects the expression of a subset of non-heat shock genes. Notably, depletion of Thoc5 attenuated the expression of the mRNAs polyadenylated at distal, but not proximal, polyadenylation sites, which phenocopied the depletion of CFIm68. Two independent experiments using different siRNAs against Thoc5 (siThoc5-1and siThoc5-2) and CFIm68 (siCFIm68-1and siCFIm68-2) were performed. In both experiments, siDsRed was used as negative control.

ORGANISM(S): Homo sapiens

SUBMITTER: Daisuke Okuzaki 

PROVIDER: E-GEOD-42327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I.

Katahira Jun J   Okuzaki Daisuke D   Inoue Hitomi H   Yoneda Yoshihiro Y   Maehara Kazumitsu K   Ohkawa Yasuyuki Y  

Nucleic acids research 20130517 14


The transcription-export complex (TREX) couples mRNA transcription, processing and nuclear export. We found that CFIm68, a large subunit of a heterotetrameric protein complex mammalian cleavage factor I (CFIm), which is implicated in alternative polyadenylation site choice, co-purified with Thoc5, a component of human TREX. Immunoprecipitation using antibodies against different components of TREX indicated that most likely both complexes interact via an interaction between Thoc5 and CFIm68. Micr  ...[more]

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