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Gene expression compare between liver cancer associated mesenchymal stem cells (LC8-MSC) and liver normal mesenchymal stem cells (LN8-MSC)

ABSTRACT: Transcriptional profiling of liver cancer associated mesenchymal stem cells comparing normal mesenchymal stem cells from adjacent tumor-free tissues of the same patient 8, Goal was to determine to detect the paracrine trophic factors from liver cancer mesenchymal stem cells. 8 represents liver cancer associated MSCs, N8 represents liver normal MSCs Two-condition experiment, 8 vs. N8 cells. Biological replicates: 1 replicate from the same patient.

ORGANISM(S): Homo sapiens

SUBMITTER: yan xin long

PROVIDER: E-GEOD-42357 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression compare between liver cancer associated mesenchymal stem cells (LC8-MSC) and liver normal mesenchymal stem cells (LN8-MSC)

Project description:Transcriptional profiling of liver cancer associated mesenchymal stem cells comparing normal mesenchymal stem cells from adjacent tumor-free tissues of the same patient 8, Goal was to determine to detect the paracrine trophic factors from liver cancer mesenchymal stem cells. 8 represents liver cancer associated MSCs, N8 represents liver normal MSCs

2012-11-17 | GSE42357 | GEO

Primary human bone marrow-derived mesenchymal stem cells (MSC) (Chip_9965581030)

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2016-12-30 | E-MTAB-5033 | biostudies-arrayexpress

Mesenchymal stem cells reduce inflammation while enhancing bacterial clearance and improving survival in sepsis.

Project description:Expression data from Total RNA extracted from murine spleen, liver, lungs, kidneys and hearts. Sepsis was induced in C57Bl/6J mice by cecal ligation and puncture (CLP), followed 6 hours later by an intravenous injection of Mesenchymal Stem Cell (MSC) or saline. Twenty-eight hours after CLP, plasma, bronchoalveolar lavage (BAL) fluid and tissues were collected for analyses. Total RNA was extracted using Trizol (as per manufactures' instruction) followed by clean-up procedure using Qiagen RNA easy Prep (as per manufactures instructions) In the following study we hypothesized that mesenchymal stem cells (MSCs), which have documented immunomodulatory properties, would reduce sepsis-associated inflammation and organ injury in a clinically relevant model of sepsis. To identify the molecular changes associated with decreased inflammation in CLP-injured mice treated with MSCs, we analyzed the gene expression profiles from spleens, liver, lungs, kidneys and heart collected at 28 hours from 4 animals per group: sham/saline, CLP/saline, and CLP/MSCs.

2012-12-31 | E-GEOD-40180 | biostudies-arrayexpress

Transcriptome profile in the human synovial MSC-aggregates

Project description:One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic potential of MSCs are needed to achieve successful cartilage regeneration by transplantation of a limited number of cells. Aggregated culture is a popular method in ES and iPS cells to maintain or enhance their potentials. Here we investigated gene expression profile of aggregated MSCs. 621 genes were up-regulated and 409 genes were down-regulated more than 5-fold in MSC-aggregates compared with the number in MSCs in a monolayer culture. The most up-regulated gene was BMP2, which is one of the genes involved in chondrogenesis. Anti-inflammatory genes were also up-regulated in MSC-aggregates. The microarray data for selected genes were confirmed by real-time PCR. Human synovial MSCs was isolated from synovium of 3 distinct donors. The gene expression profile of MSC-aggregates cultured in hanging drop for 3days was compared with that of MSCs in a monolayer culture.

2012-05-12 | E-GEOD-31980 | biostudies-arrayexpress

Mesenchymal stem cell-derived extracellular vesicles

Project description:Therapeutic benefits of mesenchymal stem/stromal cells (MSCs) are now widely believed to come from their paracrine signalling, i.e. secreted factors such as cytokines, chemokines, and extracellular vesicles (EVs). Cell-free therapy using EVs is an active and emerging field in regenerative medicine. The cellular environment of MSCs is of critical importance when directing paracrine activity. Typical 2D cultivation of stem cells on tissue culture plastic is far removed from the physiological environment of MSCs. The application of 3D cell culture allows MSCs to adapt to their cellular niche environment which, in turn, influences their paracrine signalling activity. In this study we evaluated the impact of 3D MSCs culture on EVs secretion and cargo proteome composition and functional assessment. The outcome highlights critical differences between MSC-EVs obtained from different culture microenvironments, which should be considered when scaling up MSC culture for clinical manufacturing.

2021-11-17 | PXD029766 | Pride

Homo sapiens MSCs: Normoxia vs. Hypoxia

Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.

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Expression data from MSC-treated monocytes

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2015-11-10 | E-GEOD-65561 | biostudies-arrayexpress

Transcription profiling of Mesenchymal stem cells (MSC) maintained in FCS vs autologous serum analysed at passage 4

Project description:The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs). To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) with gene expression of MSCs cultured in regular growth medium supplemented with autologous serum (AS) for 3 different donors (i.e. donor2, donor3 and donor4). MSCs were cultured in FCS- or AS-supplemented medium and were analyzed at passage 4.

2004-12-08 | E-MEXP-214 | biostudies-arrayexpress

Transcription profiling of mesenchymal stem cells (MSC) maintained in FCS or autologous serum over time analysed at passage 4 and 10

Project description:The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs)over time. To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) for 10 passages with gene expression of MSCs cultured in the same conditions for 4 passages for 2 different donors (i.e. donor3 and donor4). Likewise, we compared gene expression in MSCs maintained in regular growth medium supplemented with autologous serum(AS) for 10 passages with gene expression of MSCs cultured in the same conditions for 4 passages for the same 2 donors (i.e. donor3 and donor4). MSCs were cultured in FCS- or AS-supplemented medium and were analyzed at passage 4 and at passage 10.

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Different osteogenic differentiation behavior of canine mesenchymal stem cells derived from bone marrow and dental pulp: the proteomics profile

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2021-09-08 | PXD015512 | Pride

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