Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis in TGFbeta/TNFalpha treated A549 spheroid cultures

ABSTRACT: TGFbeta/TNFalpha treated spheroid A549 cultures are a model of the epithelial-mesenchymal transition (EMT). These experiments capture the changes in global gene expression that result from cells being induced to undergo EMT (3D control vs 3D treated), but also the differences in gene expression when A549 is grown in spheroid cultures (2D control vs 3D untreated). EMT is efficiently induced only in the spheroid culture model. A total of 8 samples are analyzed, corresponding to 4 conditions (2D control, 2D treated, 3D control, 3D treated) and 2 biological replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Marcin Cieslik

PROVIDER: E-GEOD-42373 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition.

Cieślik Marcin M Hoang Stephen A SA Baranova Natalya N Chodaparambil Sanjay S Kumar Manish M Allison David F DF Xu Xiaojiang X Wamsley J Jacob JJ Gray Lisa L Jones David R DR Mayo Marty W MW Bekiranov Stefan S

Epigenetics & chromatin 20130902 1

<h4>Background</h4>The epithelial-mesenchymal transition (EMT) is a de-differentiation process required for wound healing and development. In tumors of epithelial origin aberrant induction of EMT contributes to cancer progression and metastasis. Studies have begun to implicate epigenetic reprogramming in EMT; however, the relationship between reprogramming and the coordination of cellular processes is largely unexplored. We have previously developed a system to study EMT in a canonical non-small ...[more]

PMID: 24004852

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Dataset Information

Gene expression analysis in TGFbeta/TNFalpha treated A549 spheroid cultures

Publications

Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition.

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