Unknown,Transcriptomics,Genomics,Proteomics

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Phf19 knockdown effect on F9 embryonal carcinoma cells


ABSTRACT: PCL family protein Phf19/Pcl3 is one of the accessory components of the PRC2 core complex, and Phf19 is highly expressed in murine ES cells and an ES cell-like embryonic carcinoma cell line, F9 cells. Here we performed microarray analysis of embryonal carcinoma cell line F9 following Phf19 knockdown by shRNA. Knocking down Phf19/Pcl3 in F9 embryonic cells led to derepression of numerous PRC2 direct target genes. 4 sampels including 2 shRNA vector control cell lines and 2 shPhf19 cell lines were used for RNA extraction and Affymetrix mouse 430 2.0 arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Rui Lu 

PROVIDER: E-GEOD-42463 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.

Cai Ling L   Rothbart Scott B SB   Lu Rui R   Xu Bowen B   Chen Wei-Yi WY   Tripathy Ashutosh A   Rockowitz Shira S   Zheng Deyou D   Patel Dinshaw J DJ   Allis C David CD   Strahl Brian D BD   Song Jikui J   Wang Gang Greg GG  

Molecular cell 20121227 3


Polycomb repressive complex 2 (PRC2) regulates pluripotency, differentiation, and tumorigenesis through catalysis of histone H3 lysine 27 trimethylation (H3K27me3) on chromatin. However, the mechanisms that underlie PRC2 recruitment and spreading on chromatin remain unclear. Here we report that histone H3 lysine 36 trimethylation (H3K36me3) binding activity is harbored in the Tudor motifs of PRC2-associated polycomb-like (PCL) proteins PHF1/PCL1 and PHF19/PCL3. Ectopically expressed PHF1 induced  ...[more]

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