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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional responses to Sonic Hedgehog pathway stimulation in in vitro derived neural progenitors

ABSTRACT: The objective of this study was to identify genes regulated by Sonic Hedgehog pathway stimulation in neural progenitors. Paired (parallel culture) triplicates of neuralized embryoid body cultures with and without Shh pathway stimulation (Ag1.3-treatment)

ORGANISM(S): Mus musculus

SUBMITTER: Kevin Peterson

PROVIDER: E-GEOD-42565 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neural-specific Sox2 input and differential Gli-binding affinity provide context and positional information in Shh-directed neural patterning.

Peterson Kevin A KA Nishi Yuichi Y Ma Wenxiu W Vedenko Anastasia A Shokri Leila L Zhang Xiaoxiao X McFarlane Matthew M Baizabal José-Manuel JM Junker Jan Philipp JP van Oudenaarden Alexander A Mikkelsen Tarjei T Bernstein Bradley E BE Bailey Timothy L TL Bulyk Martha L ML Wong Wing H WH McMahon Andrew P AP

Genes & development 20121201 24

In the vertebrate neural tube, regional Sonic hedgehog (Shh) signaling invokes a time- and concentration-dependent induction of six different cell populations mediated through Gli transcriptional regulators. Elsewhere in the embryo, Shh/Gli responses invoke different tissue-appropriate regulatory programs. A genome-scale analysis of DNA binding by Gli1 and Sox2, a pan-neural determinant, identified a set of shared regulatory regions associated with key factors central to cell fate determination ...[more]

PMID: 23249739

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