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Transcriptome-wide analysis of gene expression in dark-grown WT and pif mutant seedlings

ABSTRACT: We sequenced mRNA from WT and PhytochromeInteractingFactor (pif) mutant seedlings grown for 2d in darkness. Transcriptome profiles of the wild-type (WT), pif1, pif3, pif, pif5 monogenic mutants, pif3pif4pif5 (pif345), pif1pif4pif5(pif145), pif1pif3pif5 (pif135) and pif1pif3pif4 (pif134) triple mutants and pif1pif3pif4pif5 (pifq) quadruple mutant seedlings. Biological triplicate samples were analyzed from libraries constructed using a 3'-capture, 5' to 3' directional method.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: james tepperman

PROVIDER: E-GEOD-42665 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Combinatorial complexity in a transcriptionally centered signaling hub in Arabidopsis.

Pfeiffer Anne A Shi Hui H Tepperman James M JM Zhang Yu Y Quail Peter H PH

Molecular plant 20140813 11

A subfamily of four Phytochrome (phy)-Interacting bHLH transcription Factors (PIFs) collectively promote skotomorphogenic development in dark-grown seedlings. This activity is reversed upon exposure to light, by photoactivated phy molecules that induce degradation of the PIFs, thereby triggering the transcriptional changes that drive a transition to photomorphogenesis. The PIFs function both redundantly and partially differentially at the morphogenic level in this process. To identify the direct ...[more]

PMID: 25122696

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