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Expression analysis of genes affected by dehydration-responsive transcription factor Os02g31890 from rice.

ABSTRACT: Os02g31890 encodes a dehydration-responsive transcription factor (named ´ARID´) from rice (Oryza sativa, cv. Dongjin). Expression profiling was performed 90 min after the start of dehydration stress in roots of Oryza sativa wild-type plants (cv. Dongjin) and a knock-out (i.e. arid) mutant. Wild-type rice plants and a line carrying a T-DNA insertion in the third exon of the transcription factor gene (arid mutant) were subjected to dehydration stress for 90 min. Well-watered wild-type and T-DNA insertion plants were used as controls. Total RNA was extracted from roots and subjected to expression profiling using rice Affymetrix microarrays.

ORGANISM(S): Oryza sativa

SUBMITTER: Salma Balazadeh

PROVIDER: E-GEOD-42683 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression analysis of genes affected by dehydration-responsive transcription factor Os02g31890 from rice.

Project description:Os02g31890 encodes a dehydration-responsive transcription factor (named ´ARID´) from rice (Oryza sativa, cv. Dongjin). Expression profiling was performed 90 min after the start of dehydration stress in roots of Oryza sativa wild-type plants (cv. Dongjin) and a knock-out (i.e. arid) mutant.

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Project description:Rice plants (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions with a 12-h light (28C)/12-h dark (25C) regimen (ca. 1500 umol photons mM-^V2 sM-^V1). The plants were then subjected to dehydration treatment by growth at 28C without watering for 2 days until the soil moisture content was 18.7% (w/w; SD = 1.3%).

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Wild and cultivated species of rice have distinctive proteomic responses to drought

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