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Haloarcula hispanica wild-type strain cultured in different media: MG vs 168

ABSTRACT: Transcriptional profiling of Haloarcula hispanica wild-type strain cultured in different media comparing MG medium with 168 medium. H. hispanica wild-type strain can accumulate PHBV in MG medium, while can not in 168 medium. Goal was to explore the PHA biosynthesis and its impact on central metabolism in H. hispanica. Total RNA from MG and 168 were used to generate target cDNA, and then hybridized to 8*15K Haloarcula hispanica genome array genechips, representing about 3800 genes.

ORGANISM(S): Haloarcula hispanica

SUBMITTER: Hailong Liu 

PROVIDER: E-GEOD-42798 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Proteome reference map of Haloarcula hispanica and comparative proteomic and transcriptomic analysis of polyhydroxyalkanoate biosynthesis under genetic and environmental perturbations.

Liu Hailong H   Luo Yuanming Y   Han Jing J   Wu Jinhua J   Wu Zhenfang Z   Feng Deqin D   Cai Shuangfeng S   Li Ming M   Liu Jingfang J   Zhou Jian J   Xiang Hua H  

Journal of proteome research 20130125 3

Many haloarchaea are known as polyhydroxyalkanoates (PHAs) producers, but a global and integrated view of the PHA biosynthesis is still lacking in this group of archaea. In this study, a combined proteomic and transcriptomic approach was employed in Haloarcula hispanica, a model haloarchaeon that accumulates poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) under nutrient-limiting conditions with excess carbon source. First, a comprehensive proteome reference map was established for H. hispani  ...[more]

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