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Real-time quantitative PCR analysis of mRNA from lungs of WT and Pglyrp1-/- mice sensitized with HDM

ABSTRACT: Wild type (WT) and Pglyrp1-/- mice were treated with PBS or sensitized 5 days/week for 3 or 5 weeks with 10 M-BM-5l per application of 2.5 mg/ml of purified house dust mite allergen. 3 days after the last sensitization the lungs were removed and homogenized, and RNA was isolated from the right lobes using the TRIZOL method. Quantitative reverse transcription real-time PCR (qRT-PCR) was used to quantify the amounts of mRNA in the lungs using custom RT2 Profiler PCR Arrays designed by us and manufactured by Qiagen/SA Biosciences. qRT-PCR gene expression profiling

ORGANISM(S): Mus musculus

SUBMITTER: Roman Dziarski

PROVIDER: E-GEOD-43137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Peptidoglycan recognition protein 1 enhances experimental asthma by promoting Th2 and Th17 and limiting regulatory T cell and plasmacytoid dendritic cell responses.

Park Shin Yong SY Jing Xuefang X Gupta Dipika D Dziarski Roman R

Journal of immunology (Baltimore, Md. : 1950) 20130218 7

Asthma is a common inflammatory disease involving cross-talk between innate and adaptive immunity. We reveal that antibacterial innate immunity protein, peptidoglycan recognition protein (Pglyrp)1, is involved in the development of allergic asthma. Pglyrp1(-/-) mice developed less severe asthma than wild-type (WT) mice following sensitization with house dust mite (allergen) (HDM). HDM-sensitized Pglyrp1(-/-) mice, compared with WT mice, had diminished bronchial hyperresponsiveness (lung airway r ...[more]

PMID: 23420883

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