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Functional genomic and phenotypic responses to desiccation in natural populations of a desert drosophilid.

ABSTRACT: Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (1. Punta Onah:PO; 2. Organ Pipe National Manument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ). The experiment was designed to investigate effects of desiccation exposure (0, 9 & 18 hr) and host plant (diet) on transcriptome. A total of 95 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of four populations (1. Punta Onah:PO; 2. Organ Pipe National Manument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ), two host diets (Agria and Organ pipe) and three desiccation treatments (0, 9 and 18 hours). Each chip measures the expression level of 15528 transcripts. Four to 5 replicates were used for each type (R-1, R-2, R-3 etc.)

ORGANISM(S): Drosophila mojavensis

SUBMITTER: Allen Gibbs

PROVIDER: E-GEOD-43220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional genomic and phenotypic responses to desiccation in natural populations of a desert drosophilid.

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (1. Punta Onah:PO; 2. Organ Pipe National Manument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ). The experiment was designed to investigate effects of desiccation exposure (0, 9 & 18 hr) and host plant (diet) on transcriptome.

2013-01-15 | GSE43220 | GEO

Functional genomic responses toÂ temperatureÂ variation in adultÂ Drosophila mojavensis

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (1 Punta Onah: PO, 2 Organ Pipe National Monument: OPNM, 3 Punta Prieta:PP, and 4 San Quintin: SQ). The experiment was designed to investigate functional genomic responses to temperature variation (15, 25, and 35 Â°C) in adult Drosophila mojavensis wild populations. For each treatment 1-5 replicates were used (R1, R2, R3, R4 & R5). SO and BC represents Sonora deserts and Baja California region respectively. A total of 97 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of four populations (Punta Onah:PO; Organ Pipe National Manument:OPNM, Punta Prieta:PP and San Quintin:SQ), two host diets (Agria:AG and Organ pipe:OP) and three temperature treatments (15, 25 and 35 Â°C). Each chip measures the expression level of 14528 transcripts. One to 5 replicates were used for each type (R1, R2, R3, R4 and R5). Fly source details are as follows: Punta Onah 2007:PO07; Organ Pipe National Monument 2008:OPNM08; Punta Prieta 2008:PP08; San Quintin 2008:SQ08.

2016-03-01 | E-GEOD-72169 | biostudies-arrayexpress

Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes

Project description:Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes. (1. Punta Onah:PO07; 2. Organ Pipe National Monument:OPNM08; 3. Punta Prieta:PP08; & 4. San Quintin:SQ08). The experiment was designed to investigate effects of host plant (diet), mating status (mated:M or nonmated:V) and sex (male:male or Female:F) on transcriptome. A total of 128 hybridizations were performed in this entire experiment (127 in the final analysis). We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of four populations (1. Punta Onah:PO; 2. Organ Pipe National Monument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ), two mating status (mated:M or nonmated:V), both the sexes (male:male or Female:F) fed on two different diet regimes (Agria and Organ pipe). Each chip measures the expression level of 15528 transcripts. Four to 5 replicates were used for each type (R-1, R-2, R-3 etc.)

2016-04-01 | E-GEOD-78852 | biostudies-arrayexpress

Functional genomic responses to temperature variation in adult Drosophila mojavensis

2016-03-01 | GSE72169 | GEO

Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes

2016-04-01 | GSE78852 | GEO

Preadult life history variation determines adult transcriptome expression

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate preadult life history affects over adult transcriptome expression. A total of 137 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and four "length of egg to adult development time" points, i.e. 14, 15, 16, and 17 days. (14 days:D1; 15 days:D2; 16 days:D3; 17 days:D4). Each chip measures the expression level of 14528 transcripts. One to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.

2015-08-21 | E-GEOD-72231 | biostudies-arrayexpress

Deciphering life history transcriptomics in different environments

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate life history transcriptomics in different environments. A total of 172 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and fourteen life stages (6 hr egg:E6; 1st instar larvae:L1; 2nd instar larvae:L2; 3rd instar larvae:L3; Early pupal stage:EP; Late pupal stage:LP; 0 Day old adult:0D; 3 Day old adult:3D; 4 Day old adult: 4D; 6 Day old adult:6D; 10 Day old adult:10D; 14 Day old adult:14D; 18 Day old adult:18D & 24 Day old adult:24D). Each chip measures the expression level of 14528 transcripts. Two to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.

2014-01-30 | E-GEOD-54510 | biostudies-arrayexpress

Deciphering life history transcriptomics in different environments

2014-01-30 | GSE54510 | GEO

Preadult life history variation determines adult transcriptome expression

2015-08-21 | GSE72231 | GEO