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Functional genomic and phenotypic responses to desiccation in natural populations of a desert drosophilid.

ABSTRACT: Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (1. Punta Onah:PO; 2. Organ Pipe National Manument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ). The experiment was designed to investigate effects of desiccation exposure (0, 9 & 18 hr) and host plant (diet) on transcriptome. A total of 95 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of four populations (1. Punta Onah:PO; 2. Organ Pipe National Manument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ), two host diets (Agria and Organ pipe) and three desiccation treatments (0, 9 and 18 hours). Each chip measures the expression level of 15528 transcripts. Four to 5 replicates were used for each type (R-1, R-2, R-3 etc.)

ORGANISM(S): Drosophila mojavensis

SUBMITTER: Allen Gibbs

PROVIDER: E-GEOD-43220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional genomic and phenotypic responses to desiccation in natural populations of a desert drosophilid.

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (1. Punta Onah:PO; 2. Organ Pipe National Manument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ). The experiment was designed to investigate effects of desiccation exposure (0, 9 & 18 hr) and host plant (diet) on transcriptome.

2013-01-15 | GSE43220 | GEO

Functional genomic responses toÂ temperatureÂ variation in adultÂ Drosophila mojavensis

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (1 Punta Onah: PO, 2 Organ Pipe National Monument: OPNM, 3 Punta Prieta:PP, and 4 San Quintin: SQ). The experiment was designed to investigate functional genomic responses to temperature variation (15, 25, and 35 Â°C) in adult Drosophila mojavensis wild populations. For each treatment 1-5 replicates were used (R1, R2, R3, R4 & R5). SO and BC represents Sonora deserts and Baja California region respectively. A total of 97 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of four populations (Punta Onah:PO; Organ Pipe National Manument:OPNM, Punta Prieta:PP and San Quintin:SQ), two host diets (Agria:AG and Organ pipe:OP) and three temperature treatments (15, 25 and 35 Â°C). Each chip measures the expression level of 14528 transcripts. One to 5 replicates were used for each type (R1, R2, R3, R4 and R5). Fly source details are as follows: Punta Onah 2007:PO07; Organ Pipe National Monument 2008:OPNM08; Punta Prieta 2008:PP08; San Quintin 2008:SQ08.

2016-03-01 | E-GEOD-72169 | biostudies-arrayexpress

Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes

Project description:Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes. (1. Punta Onah:PO07; 2. Organ Pipe National Monument:OPNM08; 3. Punta Prieta:PP08; & 4. San Quintin:SQ08). The experiment was designed to investigate effects of host plant (diet), mating status (mated:M or nonmated:V) and sex (male:male or Female:F) on transcriptome. A total of 128 hybridizations were performed in this entire experiment (127 in the final analysis). We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of four populations (1. Punta Onah:PO; 2. Organ Pipe National Monument:OPNM; 3. Punta Prieta:PP; & 4. San Quintin:SQ), two mating status (mated:M or nonmated:V), both the sexes (male:male or Female:F) fed on two different diet regimes (Agria and Organ pipe). Each chip measures the expression level of 15528 transcripts. Four to 5 replicates were used for each type (R-1, R-2, R-3 etc.)

2016-04-01 | E-GEOD-78852 | biostudies-arrayexpress

Functional genomic responses to temperature variation in adult Drosophila mojavensis

2016-03-01 | GSE72169 | GEO

Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes

2016-04-01 | GSE78852 | GEO

Preadult life history variation determines adult transcriptome expression

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate preadult life history affects over adult transcriptome expression. A total of 137 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and four "length of egg to adult development time" points, i.e. 14, 15, 16, and 17 days. (14 days:D1; 15 days:D2; 16 days:D3; 17 days:D4). Each chip measures the expression level of 14528 transcripts. One to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.

2015-08-21 | E-GEOD-72231 | biostudies-arrayexpress

Deciphering life history transcriptomics in different environments

Project description:Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate life history transcriptomics in different environments. A total of 172 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and fourteen life stages (6 hr egg:E6; 1st instar larvae:L1; 2nd instar larvae:L2; 3rd instar larvae:L3; Early pupal stage:EP; Late pupal stage:LP; 0 Day old adult:0D; 3 Day old adult:3D; 4 Day old adult: 4D; 6 Day old adult:6D; 10 Day old adult:10D; 14 Day old adult:14D; 18 Day old adult:18D & 24 Day old adult:24D). Each chip measures the expression level of 14528 transcripts. Two to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.

2014-01-30 | E-GEOD-54510 | biostudies-arrayexpress

Deciphering life history transcriptomics in different environments

2014-01-30 | GSE54510 | GEO

Preadult life history variation determines adult transcriptome expression

2015-08-21 | GSE72231 | GEO

Expression data from adipocyte differentiation from diabetic adipose-derived stem cells (dADSC) treated with squalene (Sq) and its derivative (HH-Sq)

Project description:Gene expression profiling reveals functional difference between Sq and HH-Sq on differentiation, metabolism, and lipid droplot formation of dADSC Microarray gene expression profiling was conducted for technical replicates of adipocyte induced dADSC (IN), adipocyte induced and treated with Sq (Sq), adipocyte induced and treated with HH-Sq (HH-Sq) for 14 days (1 µM) to identify its effect in the regulation of adipocyte differentiation, metabolism, and lipid droplet accumulation. Technical replicates represent the same hybridization mixture applied to 3 independent arrays.

2020-06-27 | GSE153391 | GEO

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