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Genome wide-DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients (methylation)

ABSTRACT: The aim of this study is to identify, for the first time, the genome-wide DNA methylation profiles of human articular chondrocytes from OA and healtly cartilage samples. Genome wide DNA methylation profiling of normal and osteoarthritic samples. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in cartilage knee samples. Samples included 18 healthy controls and 23 OA patients. Bisulphite converted DNA from the 31 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2

ORGANISM(S): Homo sapiens

SUBMITTER: Juan Fernandez Tajes

PROVIDER: E-GEOD-43269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients.

Fernández-Tajes Juan J Soto-Hermida Angel A Vázquez-Mosquera Maria E ME Cortés-Pereira Estefania E Mosquera Alejandro A Fernández-Moreno Mercedes M Oreiro Natividad N Fernández-López Carlos C Fernández Jose Luis JL Rego-Pérez Ignacio I Blanco Francisco J FJ

Annals of the rheumatic diseases 20130316 4

<h4>Objective</h4>Alterations in DNA methylation patterns have been found to correlate with several diseases including osteoarthritis (OA). The aim of this study was to identify, for the first time, the genome-wide DNA methylation profiles of human articular chondrocytes from OA cartilage and healthy control cartilage samples.<h4>Methods</h4>DNA methylation profiling was performed using Illumina Infinium HumanMethylation27 in 25 patients with OA and 20 healthy controls. Subsequent validation was ...[more]

PMID: 23505229

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