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Identification of in vivo, conserved, TAF15 RNA binding sites and the impact of TAF15 on the neuronal transcriptome

ABSTRACT: TAF15, an RNA binding protein was recently implicated in Amyotrophic Lateral Sclerosis (ALS). ALS is a fatal neurodegenerative disease. We report the identification of the conserved neuronal RNA targets of TAF15 and the assessment of the impact of TAF15 depletion on the neuronal transcriptome. Our study uncovers regulation of splicing of sets of neuronal RNAs encoding proteins with essential roles in synaptic activities including glutamergic receptors such as zeta-1 subunit of the glutamate N-methyl-D-aspartate (NMDA) receptor (Grin1). Identification of TAF15 neuronal targets using normal human brain samples and mouse neurons. Mouse background: E14Tg2a.4 wildtype cells derived from 129P2/OlaHsd.

ORGANISM(S): Mus musculus

SUBMITTER: Manolis Maragkakis

PROVIDER: E-GEOD-43294 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of in vivo, conserved, TAF15 RNA binding sites reveals the impact of TAF15 on the neuronal transcriptome.

Ibrahim Fadia F Maragkakis Manolis M Alexiou Panagiotis P Maronski Margaret A MA Dichter Marc A MA Mourelatos Zissimos Z

Cell reports 20130214 2

RNA binding proteins (RBPs) have emerged as major causative agents of amyotrophic lateral sclerosis (ALS). To investigate the function of TAF15, an RBP recently implicated in ALS, we explored its target RNA repertoire in normal human brain and mouse neurons. Coupling high-throughput sequencing of immunoprecipitated and crosslinked RNA with RNA sequencing and TAF15 knockdowns, we identified conserved TAF15 RNA targets and assessed the impact of TAF15 on the neuronal transcriptome. We describe a r ...[more]

PMID: 23416048

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