Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiling of lung adenocarcinomas and normal lung tissue

ABSTRACT: Lung cancer is still the leading cause of cancer-related deaths in the US and worldwide. Understanding the global molecular profiles or transcriptome of lung cancers would strengthen our understanding of the biology of this malignancy. We performed gene expression profiling using the Human Gene 1.0 ST platform of 80 lung adenocarcinomas and 30 normal lung tissues to better understand the biology of this significant fraction of non-small cell lung carcinomas (NSCLCs) Lung adenocarcinomas were comrpised of never-smoker (n=40) and smoker (n=40) adenocarcinomas. Normal lung tissue (n=30) were paired to 30 of the never-smoker cases. Gene expression profiling was performed on the samples to identify differentially expressed profiles between lung adenocarcinomas and normal lung tissues.

ORGANISM(S): Homo sapiens

SUBMITTER: Humam Kadara

PROVIDER: E-GEOD-43458 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ETS2 mediated tumor suppressive function and MET oncogene inhibition in human non-small cell lung cancer.

Kabbout Mohamed M Garcia Melinda M MM Fujimoto Junya J Liu Diane D DD Woods Denise D Chow Chi-Wan CW Mendoza Gabriela G Momin Amin A AA James Brian P BP Solis Luisa L Behrens Carmen C Lee J Jack JJ Wistuba Ignacio I II Kadara Humam H

Clinical cancer research : an official journal of the American Association for Cancer Research 20130509 13

<h4>Purpose</h4>The ETS2 transcription factor is an evolutionarily conserved gene that is deregulated in cancer. We analyzed the transcriptome of lung adenocarcinomas and normal lung tissue by expression profiling and found that ETS2 was significantly downregulated in adenocarcinomas. In this study, we probed the yet unknown functional role of ETS2 in lung cancer pathogenesis.<h4>Experimental design</h4>Lung adenocarcinomas (n = 80) and normal lung tissues (n = 30) were profiled using the Affyme ...[more]

PMID: 23659968

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Project description:Approximately 15% of lung cancer cases are not associated with smoking and show molecular and clinical characteristics distinct from those in smokers. Epidermal growth factor receptor (EGFR) gene mutations, which are correlated with sensitivity to EGFR-tyrosine kinase inhibitors (EGFR-TKIs), are more frequent in never-smoker lung cancers. In this study, microRNA (miRNA) expression profiling of 28 never-smoker lung cancer cases identified aberrantly expressed miRNAs, which were much fewer than in lung cancers of smokers and included miRNAs previously identified (e.g., upregulated miR-21) and unidentified (e.g., downregulated miR-138) in those smoker cases. The changes in expression of some of these miRNAs were more remarkable in cases with EGFR mutations than in those without: the most upregulated miRNA, miR-21, was more abundant in cancers with EGFR mutation. A significant correlation between phosphorylated-EGFR (p-EGFR) and miR-21 levels in lung carcinoma cell lines and the suppression of miR-21 by an EGFR-TKI, AG1478, suggested that the EGFR signaling pathway positively regulated miR-21 expression. In a never-smoker-derived lung adenocarcinoma cell line H3255 with mutant EGFR and high levels of p-EGFR and miR-21, antisense inhibition of miR-21 enhanced AG1478-induced apoptosis. In a never-smoker-derived adenocarcinoma cell line H441 with wild-type EGFR, the antisense miR-21 not only showed the additive effect with AG1478 but also induced apoptosis by itself. These results suggest that aberrantly increased expression of miR-21, which is further enhanced by the activated EGFR signaling pathway, plays a critical role in lung carcinogenesis in never-smokers and is a potential therapeutic target in both EGFR mutant and wild-type cases. Twenty-eight pairs of lung cancer tissues and corresponding noncancerous lung tissues were obtained from never-smokers who had undergone surgical resection from 2000 to 2004 at the University of Maryland Medical Center (n=15), Mayo Clinic (n=7) in United States and Hamamatsu University School of Medicine (n=6) in Japan.

Dataset Information

Gene expression profiling of lung adenocarcinomas and normal lung tissue

Publications

ETS2 mediated tumor suppressive function and MET oncogene inhibition in human non-small cell lung cancer.

Similar Datasets

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