Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The global gene expression profile of superficial zone articular cartilage in P1 wildtype and Prg4 transgenic mice

ABSTRACT: To examine the unbiased global gene expression of over-expression of Prg4 in superficial zone articular chondrocytes in mice. This approach allows us to define novel signaling changes caused by the over-expression of Prg4 in superficial zone articular chondrocytes Total RNA was isolated from superficial zone chondrocytes in P1 articular cartilage in hind limbs (n=3) by laser capture microdissection. Same tissue from wild type mice served as control

ORGANISM(S): Mus musculus

SUBMITTER: Merry Ruan

PROVIDER: E-GEOD-43663 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Proteoglycan 4 expression protects against the development of osteoarthritis.

Ruan Merry Z C MZ Erez Ayelet A Guse Kilian K Dawson Brian B Bertin Terry T Chen Yuqing Y Jiang Ming-Ming MM Yustein Jason J Gannon Francis F Lee Brendan H L BH

Science translational medicine 20130301 176

Osteoarthritis (OA) is a common degenerative condition that afflicts more than 70% of the population between 55 and 77 years of age. Although its prevalence is rising globally with aging of the population, current therapy is limited to symptomatic relief and, in severe cases, joint replacement surgery. We report that intra-articular expression of proteoglycan 4 (Prg4) in mice protects against development of OA. Long-term Prg4 expression under the type II collagen promoter (Col2a1) does not adver ...[more]

PMID: 23486780

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Project description:Articular and growth plate cartilage have comparable structures consisting of three distinct layers of chondrocytes, suggesting similar differentiation programs and therefore similar gene expression profiles. To address this hypothesis and to explore transcriptional changes that occur during the onset of articular and growth plate cartilage divergence, we used microdissection of 10-day-old rat proximal tibial epiphyses, microarray analysis, and bioinformatics to compare gene expression profiles in individual layers of articular and growth plate cartilage. We found that many genes that were spatially upregulated in intermediate/deep zone of articular cartilage were also spatially upregulated in resting zone of growth plate cartilage (overlap greater than expected by chance, P < 0.001). Interestingly, superficial zone of articular cartilage showed an expression profile with similarities to both proliferative and hypertrophic zones of growth plate cartilage (P < 0.001 each). Additionally, significant numbers of known proliferative zone markers (3 out of 6) and hypertrophic zone markers (27 out of 126) were spatially upregulated in superficial zone compared to intermediate/deep zone (more than expected by chance, P < 0.001 each). In conclusion, we provide evidence that intermediate/deep zone of articular cartilage has a gene expression profile more similar to resting zone of growth plate cartilage, whereas superficial zone has a gene expression profile more similar to proliferative and hypertrophic zones. 10-day-old rat proximal tibial epiphyses were manually microdissected into articular cartilage superficial (SZ) and intermediate/deep (IDZ) zones and growth plate cartilage resting zone (RZ) for total RNA extraction and hybridization on Affymetrix microarrays. We used 10-day-old animals because, at this age, the secondary ossification center has recently begun to form and divides the epiphysis into articular cartilage distally and growth plate cartilage more centrally. The 4 SZ samples were taken from animals 5-8, respectively, whereas the 4 IDZ and 4 RZ samples were each taken from animals 1-2, 3-4, 5-6, and 7-8, respectively.

Dataset Information

The global gene expression profile of superficial zone articular cartilage in P1 wildtype and Prg4 transgenic mice

Publications

Proteoglycan 4 expression protects against the development of osteoarthritis.

Similar Datasets

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