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A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme


ABSTRACT: The histone variant H2A.Z plays key roles in gene expression, DNA repair, and centromere function. H2A.Z deposition is controlled by SWR-C chromatin remodeling enzymes that catalyze the nucleosomal exchange of canonical H2A with H2A.Z. Here we report that acetylation of histone H3 lysine 56 (H3-K56Ac) alters the substrate specificity of SWR-C, leading to promiscuous dimer exchange where either H2A.Z or H2A can be exchanged from nucleosomes. This result is confirmed in vivo, where genome-wide analysis demonstrates widespread decreases in H2A.Z levels in yeast mutants with hyperacetylated H3K56. Our work also suggests that a conserved SWR-C subunit may function as a M-bM-^@M-^\lockM-bM-^@M-^] that prevents removal of H2A.Z from nucleosomes. Our study identifies a histone modification that regulates a chromatin remodeling reaction and provides insights into how histone variants and nucleosome turnover can be controlled by chromatin regulators. H2A.Z ChIP seq experiments in mutants with constitutive H3K56ac

ORGANISM(S): Saccharomyces cerevisiae W303

SUBMITTER: Marta Radman-Livaja 

PROVIDER: E-GEOD-43935 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme.

Watanabe Shinya S   Radman-Livaja Marta M   Rando Oliver J OJ   Peterson Craig L CL  

Science (New York, N.Y.) 20130401 6129


The histone variant H2A.Z plays key roles in gene expression, DNA repair, and centromere function. H2A.Z deposition is controlled by SWR-C chromatin remodeling enzymes that catalyze the nucleosomal exchange of canonical H2A with H2A.Z. Here we report that acetylation of histone H3 on lysine 56 (H3-K56Ac) alters the substrate specificity of SWR-C, leading to promiscuous dimer exchange in which either H2A.Z or H2A can be exchanged from nucleosomes. This result was confirmed in vivo, where genome-w  ...[more]

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