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Upregulation of ERBB3 by FOXD3, an adaptive response to RAF and MEK inhibitors

ABSTRACT: To understand the transcriptional impact of FOXD3 in melanoma cells, we utilized a microarray approach. We collected RNA from three unrelated mutant B-RAF melanoma cell lines (WM115, WM793, and A375) that were engineered to inducibly express FOXD3 or the control gene, ?-galactosidase (LacZ), after 5 days of transgene induction. This time point was chosen based on maximal phenotypic changes previously observed. Comparison of gene signatures between the 3 cell lines produced approximately 2,600 common genes differentially regulated by FOXD3-expressing cells compared to the LacZ controls. Three unrelated mutant B-RAF melanoma cell lines (WM115, WM793, and A375) were induced to express FOXD3 and compared against the same cell lines expressing the control gene, ?-galactosidase (LacZ).

ORGANISM(S): Homo sapiens

SUBMITTER: Adam Ertel

PROVIDER: E-GEOD-43962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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