Unknown,Transcriptomics,Genomics,Proteomics

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Studies of target genes for signal transduction pathways (using a Ras1-, a Cdc42- and a Gap1-mutant) in the basidiomycete Schizophyllum commune.


ABSTRACT: Transcriptome changes associated with signal transduction mutants were analysed in order to identify targets of the distinct pathways of Ras1, Cdc42 and Gap1. Two monokaryons (12-43 and 4-39), a dikaryon (W22 x 12-43), a constitutive active Ras1 mutant (T2G12V, monokaryon), a Cdc42 mutant (G12V, monokaryon) and a M-bM-^HM-^Fgap1 x M-bM-^HM-^Fgap1 dikaryon (F15 x F28, deletion of Gap1) were analyzed for that purpose. With microarray analysis mRNA expression in different mutants of the basidiomycete S. commune was analyzed. Genes which show regulation in the comparison either of the two monokaryons 12-43 and 4-39 (for Ras2 and Cdc42 mutant) or the dikaryon W22 x 12-43 (for Gap1 x Gap1 dikaryon) have been eliminated from other comparisons as stage-specific (mono- or dikaryon) differences. Two biological replicate samples were analyzed for each condition.

ORGANISM(S): Schizophyllum commune

SUBMITTER: Erika Kothe 

PROVIDER: E-GEOD-43965 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A central role for Ras1 in morphogenesis of the basidiomycete Schizophyllum commune.

Knabe Nicole N   Jung Elke-Martina EM   Freihorst Daniela D   Hennicke Florian F   Horton J Stephen JS   Kothe Erika E  

Eukaryotic cell 20130419 6


Fungi have been used as model systems to define general processes in eukaryotes, for example, the one gene-one enzyme hypothesis, as well as to study polar growth or pathogenesis. Here, we show a central role for the regulator protein Ras in a mushroom-forming, filamentous basidiomycete linking growth, pheromone signaling, sexual development, and meiosis to different signal transduction pathways. ras1 and Ras-specific gap1 mutants were generated and used to modify the intracellular activation st  ...[more]

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