Unknown,Transcriptomics,Genomics,Proteomics

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MiR-223 in esophageal adenocarcinoma carcinogenesis

ABSTRACT: miR-223 is step-wise increasingly up-regulated in the normal esophagus - Barrett's esophagus -esophageal adenocarcinoma carcinoma sequence. In this study, we aimed to determine the function of miR-223 in esophageal adenocarcinoma carcinogenesis. miR-223 was transfected in OE33 cells using 10nM pre-miR hsa-miR-223 miRNA precursor (Ambion, Life Technologies, Grand Island, NY) and lipofectamin 2000 (OE33_223_1 and OE33_223_2). Mock control OE33 cells were transfected with a negative control pre-miR miRNA (OE33_NEG_1 and OE33_NEG_2). HumanHT-12 v4 Expression BeadChip arrays (Illumina, San Diego, CA) were used for microarray hybridizations to examine the global gene expression of two biological replicated experiments (four samples in total). The array targets more than 25,000 annotated genes with 47,323 unique probes derived from the National Center for Biotechnology Information (NCBI) Reference Sequence (RefSeq) Release 38 and UniGene (Build 199) databases.

ORGANISM(S): Homo sapiens

SUBMITTER: Mirte Streppel 

PROVIDER: E-GEOD-44120 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

MicroRNA 223 is upregulated in the multistep progression of Barrett's esophagus and modulates sensitivity to chemotherapy by targeting PARP1.

Streppel Mirte Mayke MM   Pai Shweta S   Campbell Nathaniel R NR   Hu Chaoxin C   Yabuuchi Shinichi S   Canto Marcia Irene MI   Wang Jean S JS   Montgomery Elizabeth A EA   Maitra Anirban A  

Clinical cancer research : an official journal of the American Association for Cancer Research 20130611 15

<h4>Purpose</h4>Recent microarray and RNA-sequencing studies have uncovered aberrantly expressed microRNAs (miRNA) in Barrett's esophagus-associated esophageal adenocarcinoma. The functional significance of these miRNAs in esophageal adenocarcinoma initiation and progression is largely unknown.<h4>Experimental design</h4>Expression levels of miR-199a/b-3p, -199a-5p, -199b-5p, -200b, -200c, -223, and -375 were determined in microdissected tissues from cardiac mucosa, Barrett's esophagus, dysplast  ...[more]

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