Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in Bacillus subtilis treated with human PGRP, gentamicin, and CCCP

ABSTRACT: Human Peptidoglycan Recognition Proteins (PGRPs) kill bacteria, likely by over-activating stress responses in bacteria. To gain insight into the mechanism of PGRP killing of Bacillus subtilis and bacterial defense against PGRP killing, gene expression in B. subtilis treated with a control protein (bovine serum albumin, BSA), human recombinant PGRP (PGLYRP4), gentamicin (aminoglycoside antibiotic), and CCCP (membrane potential decoupler) were compared. Each treatment induced unique and somewhat overlapping pattern of gene expression. PGRP highly increased expression of genes for oxidative and disulfide stress, detoxification and efflux of Cu, As, and Zn, several transporters, repair of damaged proteins and DNA, energy generation, histidine and cysteine synthesis, envelope lysis and remodeling, and other stress responses. PGRP also caused marked decrease in the expression of genes for phosphate uptake and utilization, Fe uptake, and motility. Gene expression microarray in B. subtilis exposed to a human bactericidal innate immunity protein, PGRP, showed induction of oxidative stress response and defense genes, with different expression pattern than B. subtilis exposed to an aminoglycoside antibiotic and a membrane potential decoupler. B. subtilis was treated with PGRP (human recombinant PGLYRP4), gentamicin, or CCCP (carbonyl cyanide 3-chlorophenylhydrazone). RNA was obtained from each culture and assayed for gene expression using custom whole genome Affymetrix 900513 GeneChip B. subtilis Genome Array. Each experiment was repeated 3 times.

ORGANISM(S): Bacillus subtilis

SUBMITTER: Roman Dziarski

PROVIDER: E-GEOD-44212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Peptidoglycan recognition proteins kill bacteria by inducing oxidative, thiol, and metal stress.

Kashyap Des Raj DR Rompca Annemarie A Gaballa Ahmed A Helmann John D JD Chan Jefferson J Chang Christopher J CJ Hozo Iztok I Gupta Dipika D Dziarski Roman R

PLoS pathogens 20140717 7

Mammalian Peptidoglycan Recognition Proteins (PGRPs) are a family of evolutionary conserved bactericidal innate immunity proteins, but the mechanism through which they kill bacteria is unclear. We previously proposed that PGRPs are bactericidal due to induction of reactive oxygen species (ROS), a mechanism of killing that was also postulated, and later refuted, for several bactericidal antibiotics. Here, using whole genome expression arrays, qRT-PCR, and biochemical tests we show that in both Es ...[more]

PMID: 25032698

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Dataset Information

Gene expression in Bacillus subtilis treated with human PGRP, gentamicin, and CCCP

Publications

Peptidoglycan recognition proteins kill bacteria by inducing oxidative, thiol, and metal stress.

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