Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Real-time quantitative PCR analysis of human astrocytomas


ABSTRACT: Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not children. We examined the global protein and microRNAs expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF) and RT2 miRNA PCR Array System. MicroRNAs analysis revealed for the first time novel microRNAs involved in astrocytomas biology. Interestingly, miR-138 and miR-145 down-regulation appear to be associated with protein over-expression of vimentin and Bax, respectively. In conclusion, our results show that novel proteins and microRNAs altered on pediatric astrocytoma could serve as biomarkers to distinguish between astrocytoma grades. Astrocytoma samples were colected from patients and total RNA isolation (30 mg of tissue) was performed using the TRIzolM-BM-. protocol (Invitrogen, USA) according to the manufacturerM-bM-^@M-2s instructions. Samples were analyzed using SA Biosciences RT2 miRNA PCRArray System to determied the miRNA expression between control samples and tumors RT2 miRNA PCR Array. Eigth tumor samples and two control tissue (including two control tissue replicates) were used as indicated in the sumary. A total of 3ug of RNA from each tumr samples and control tissue were placed in the PCR Array

ORGANISM(S): Homo sapiens

SUBMITTER: Ruth Ruiz-Esparza 

PROVIDER: E-GEOD-44280 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF), and RT(2) miRNA PCR Array System. Proteomic studies revealed 49 proteins with changes on the expression. Interactome showed that vimentin, calreticulin, and 14-3-3 epsilon prote  ...[more]

Similar Datasets

2013-02-12 | GSE44280 | GEO
2012-01-01 | E-GEOD-34142 | biostudies-arrayexpress
2012-04-01 | E-GEOD-34827 | biostudies-arrayexpress
2013-01-15 | E-GEOD-43469 | biostudies-arrayexpress
2012-01-01 | GSE34142 | GEO
2012-04-01 | GSE34827 | GEO
2014-02-28 | E-GEOD-55427 | biostudies-arrayexpress
2017-07-03 | GSE74567 | GEO
2012-04-30 | E-GEOD-37671 | biostudies-arrayexpress
2015-10-30 | E-GEOD-73066 | biostudies-arrayexpress