Reprogramming to pluripotency through a somatic stem cell intermediate

ABSTRACT: In the past years, transdifferentiation from one lineage to another has become an important research field. In that context, we recently reported the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced neural stem cells (iNSCs). In this study, we, for the first time, successfully reprogrammed these iNSCs into induced pluripotent stem cells (iPSCs), which we termed iNSC-derived iPSCs (iNdiPSCs). iNdiPSCs proved to be truly pluripotent, as shown by a pluripotent gene expression profile and the ability to differentiate into all three germ layers both, in vitro and in vivo. We could further show that iNdiPSCs do not retain an epigenetic memory neither from iNSCs nor MEFs. In conclusion, our data validate the stable somatic stem cell status of iNSCs and demonstrate their pot

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-44284 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress