Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis thaliana response to the bacterial pathogen Pseudomonas syringae ES4326


ABSTRACT: In order to protect themselves from pathogens, plants activate a battery of defense pathways, many of which involve changes in gene expression. We are interested in identifying plant genes that are differentially expressed in response to pathogen exposure, with the ultimate goal of studying the roles of these genes in plant defense. Experiment Overall Design: Whole plants are grown in soil, and individual leaves from 3-4 week old plants are injected with a bacterial suspension, or a mock (5mM MgSO4) control. Approximately five leaves are harvested 24 hours after inoculation and used for RNA preparation, labeling and Affymetrix hybridization.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Raka Mustaphi Mitra 

PROVIDER: E-GEOD-4429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A high-performance, small-scale microarray for expression profiling of many samples in Arabidopsis-pathogen studies.

Sato Masanao M   Mitra Raka M RM   Coller John J   Wang Dong D   Spivey Natalie W NW   Dewdney Julia J   Denoux Carine C   Glazebrook Jane J   Katagiri Fumiaki F  

The Plant journal : for cell and molecular biology 20061220 3


Studies of the behavior of biological systems often require monitoring of the expression of many genes in a large number of samples. While whole-genome arrays provide high-quality gene-expression profiles, their high cost generally limits the number of samples that can be studied. Although inexpensive small-scale arrays representing genes of interest could be used for many applications, it is challenging to obtain accurate measurements with conventional small-scale microarrays. We have developed  ...[more]

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