Unknown,Transcriptomics,Genomics,Proteomics

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Effect of PCO1 overexpression [hypoxia]

ABSTRACT: The effect of the overexpression of Plant Cysteine Oxidase (PDCO1) on the transcriptome of Arabidopsis resettes was investigated with plants subjected to a 4h hypoxia (5% O2 v/v in air). For this purpose, 4-week old rosette of wild-type and 35S:FLAG:CDO1 plants were compared. Samples were composed of pools of 5 plants. In total, 6 samples were analyzes, subdivided in 2 thesis with 3 replicates each. 35S:PCO1 plants (treated with hypoxia) were compared to wild-type plants (treated with hypoxia).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Francesco Licausi

PROVIDER: E-GEOD-44343 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway.

Weits Daan A DA Giuntoli Beatrice B Kosmacz Monika M Parlanti Sandro S Hubberten Hans-Michael HM Riegler Heike H Hoefgen Rainer R Perata Pierdomenico P van Dongen Joost T JT Licausi Francesco F

Nature communications 20140306

In plant and animal cells, amino-terminal cysteine oxidation controls selective proteolysis via an oxygen-dependent branch of the N-end rule pathway. It remains unknown how the N-terminal cysteine is specifically oxidized. Here we identify plant cysteine oxidase (PCO) enzymes that oxidize the penultimate cysteine of ERF-VII transcription factors by using oxygen as a co-substrate, thereby controlling the lifetime of these proteins. Consequently, ERF-VII proteins are stabilized under hypoxia and a ...[more]

PMID: 24599061

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Dataset Information

Effect of PCO1 overexpression [hypoxia]

Publications

Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway.

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